MiR-155 inhibits proliferation and invasion by directly targeting PDCD4 in non-small cell lung cancer.
Thorac Cancer
; 8(6): 613-619, 2017 11.
Article
em En
| MEDLINE
| ID: mdl-28842954
ABSTRACT
BACKGROUND:
MicroRNAs are often abnormally expressed in human non-small cell lung cancer (NSCLC) and are thought to play a critical role in the emergence or maintenance of NSCLC by binding to its target messenger RNA. We assessed the effects of miR-155 on cell proliferation and invasion to elucidate the role played by miR-155/PDCD4 in NSCLC.METHODS:
Quantitative reverse transcription-PCR, Western blotting, and cell counting kit-8, luciferase, and transwell invasion assays were conducted on a normal human bronchial epithelial cell line (BEAS-2B) and three NSCLC cell lines (SPC-A-1, A549, and H2170).RESULTS:
We confirmed that miR-155 was upregulated, while PDCD4 messenger RNA and protein levels were downregulated in NSCLC cell lines. miR-155 negatively regulated PDCD4 at both transcriptional and post-transcriptional levels. Moreover, PDCD4 was forecast as an assumed target of miR-155 using bioinformatic methods and we demonstrated that PDCD4 was a direct target of miR-155 using luciferase reporter assays. Furthermore, PDCD4 overexpression could restrain NSCLC proliferation and invasion induced by miR-155.CONCLUSION:
Our results collectively demonstrate that miR-155 exerts an oncogenic role in NSCLC by directly targeting PDCD4.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Ligação a RNA
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Carcinoma Pulmonar de Células não Pequenas
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MicroRNAs
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Proteínas Reguladoras de Apoptose
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Neoplasias Pulmonares
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Thorac Cancer
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
China