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[Role of PpIX-based photodynamic therapy in promoting the damage and apoptosis of colorectal cancer cell and its mechanisms].
Ouyang, Guoqing; Liu, Zhipeng; Xiong, Li; Chen, Xiang; Li, Qinglong; Huang, He; Lin, Liangwu; Miao, Xiongying; Ma, Lun; Chen, Wei; Wen, Yu.
Afiliação
  • Ouyang G; Department of General Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China.
  • Liu Z; Department of General Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China.
  • Xiong L; Department of General Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China.
  • Chen X; Department of General Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China.
  • Li Q; Department of General Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China.
  • Huang H; Department of Histology and Embryology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
  • Lin L; Powder Metallurgy Research Institute, Central South University, Changsha 410083, China.
  • Miao X; Department of General Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China.
  • Ma L; Department of Physics, University of Texas at Arlington, Arlington Texas 76019-0059, USA.
  • Chen W; Department of General Surgery, Second Xiangya Hospital, Central South University, Changsha 410011; Department of Physics, University of Texas at Arlington, Arlington Texas 76019-0059, USA.
  • Wen Y; Department of General Surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 42(8): 874-881, 2017 Aug 28.
Article em Zh | MEDLINE | ID: mdl-28872077
OBJECTIVE: To explore the effects of protoporphyrin IX (PpIX)-mediated photodynamic therapy (PDT) on induction of apoptosis and death in colon cancer cell and the underlying mechanisms.
 Methods: The cell killing effect of PDT on HCT116 cell was determined by cell counting kit (CCK). The cells were divided into a control group, a single light group, a single PpIX group, and a PDT group. Hoechst 33342 and flow cytometry was used to assess the cell apoptosis. Western blot was employed to analyze the expressions of bcl-2, bax, and caspase-3. Reactive oxygen species (ROS) was detected by flow cytometry.
 Results: The viability of HCT116 cell was decreased gradually with the increase of irradiation dose (P<0.05). Compared to the other 3 groups, ROS production, the number of apoptotic cells and the protein expressions of bax and caspase-3 in PDT group increased, while bcl-2 expression was decreased (P<0.05).
 Conclusion: PpIX-mediated PDT can enhance the apoptosis in HCT116 cell, which may be related to mitochondrial apoptosis pathway.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fotoquimioterapia / Apoptose Limite: Humans Idioma: Zh Revista: Zhong Nan Da Xue Xue Bao Yi Xue Ban Assunto da revista: MEDICINA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: China
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fotoquimioterapia / Apoptose Limite: Humans Idioma: Zh Revista: Zhong Nan Da Xue Xue Bao Yi Xue Ban Assunto da revista: MEDICINA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: China