Viscosity reduction of isotonic solutions of the photosensitizer TPCS2a by cyclodextrin complexation.
Drug Dev Ind Pharm
; 44(2): 261-265, 2018 Feb.
Article
em En
| MEDLINE
| ID: mdl-28956453
ABSTRACT
Meso-tetraphenyl chlorin disulphonate (TPCS2a) is a photosensitizer (PS) particularly developed and patented for use in the technology of photochemical internalization (PCI) against cancer. TPCS2a is known to aggregate in aqueous media even at low concentrations (≥0.1 µM) and to form a high-viscosity network at clinically relevant concentrations (mM). The aim of this work was to evaluate the effect of two hydroxypropylated cyclodextrin derivatives of beta and gamma type, respectively i.e. HPßCD and HPγCD, on the aggregation and solubilization of TPCS2a in isotonic solutions. Samples containing micromolar concentrations of TPCS2a were studied spectrophotometrically, while samples containing a clinical relevant concentration (10 mM = 9 mg/ml) of TPCS2a were evaluated by dynamic viscosity measurements. HPßCD was determined to be a more suitable solubilizer of TPCS2a than HPγCD in aqueous media both in the absence and presence of salt. The complexation stoichiometry between TPCS2a/HPßCD at micromolar to millimolar concentrations of TPCS2a was determined to be 13 and 12 in the absence and presence of isotonic NaCl, respectively. The network of TPCS2a (10 mM) was broken down in the presence of 3% w/v (= 20 mM) HPßCD, i.e. a 12 molar ratio between TPCS2a and the cyclodextrin. Formation of the inclusion complex resulted in low viscosity samples both in water and in the presence of isotonic NaCl or phosphate buffered saline (PBS) at 25 °C and 37 °C.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Porfirinas
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Fármacos Fotossensibilizantes
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Beta-Ciclodextrinas
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Gama-Ciclodextrinas
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Soluções Isotônicas
Idioma:
En
Revista:
Drug Dev Ind Pharm
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Noruega