ADP-ribosyltransferase activity of pertussis toxin and immunomodulation by Bordetella pertussis.
Science
; 240(4852): 656-9, 1988 Apr 29.
Article
em En
| MEDLINE
| ID: mdl-2896387
ABSTRACT
Pertussis toxin is produced by the causative agent of whooping cough, Bordetella pertussis, and is an adenosine diphosphate (ADP)-ribosyltransferase capable of covalently modifying and thereby inactivating many eukaryotic G proteins involved in cellular metabolism. The toxin is a principal determinant of virulence in whooping cough and is a primary candidate for an acellular pertussis vaccine, yet it is unclear whether the ADP-ribosyltransferase activity is required for both pathogenic and immunoprotective activities. A B. pertussis strain that produced an assembled pertussis holotoxin with only 1 percent of the ADP-ribosyltransferase activity of the native toxin was constructed and was found to be deficient in pathogenic activities associated with B. pertussis including induction of leukocytosis, potentiation of anaphylaxis, and stimulation of histamine sensitivity. Moreover, this mutant strain failed to function as an adjuvant and was less effective in protecting mice from intracerebral challenge infection. These data suggest that the ADP-ribosyltransferase activity is necessary for both pathogenicity and optimum immunoprotection. These findings bear directly on the design of a nontoxic pertussis vaccine.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pentosiltransferases
/
Bordetella pertussis
/
Fatores de Virulência de Bordetella
/
Toxina Pertussis
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Revista:
Science
Ano de publicação:
1988
Tipo de documento:
Article