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Repeated Oral Exposure to N ε-Carboxymethyllysine, a Maillard Reaction Product, Alleviates Gut Microbiota Dysbiosis in Colitic Mice.
ALJahdali, Nesreen; Gadonna-Widehem, Pascale; Delayre-Orthez, Carine; Marier, David; Garnier, Benjamin; Carbonero, Franck; Anton, Pauline M.
Afiliação
  • ALJahdali N; Cell and Molecular Biology Program, University of Arkansas, 2650 Young Avenue, Fayetteville, AR, 72704, USA.
  • Gadonna-Widehem P; Expression des Gènes et Régulation Epigénétique par l'Aliment UP 2012.10.101., Institut Polytechnique UniLaSalle, 19 rue Pierre Waguet, 60000, Beauvais, France.
  • Delayre-Orthez C; Expression des Gènes et Régulation Epigénétique par l'Aliment UP 2012.10.101., Institut Polytechnique UniLaSalle, 19 rue Pierre Waguet, 60000, Beauvais, France.
  • Marier D; Expression des Gènes et Régulation Epigénétique par l'Aliment UP 2012.10.101., Institut Polytechnique UniLaSalle, 19 rue Pierre Waguet, 60000, Beauvais, France.
  • Garnier B; Expression des Gènes et Régulation Epigénétique par l'Aliment UP 2012.10.101., Institut Polytechnique UniLaSalle, 19 rue Pierre Waguet, 60000, Beauvais, France.
  • Carbonero F; Cell and Molecular Biology Program, University of Arkansas, 2650 Young Avenue, Fayetteville, AR, 72704, USA. fgcarbon@uark.edu.
  • Anton PM; Department of Food Science and Center for Human Nutrition, University of Arkansas, 2650 Young Avenue, Fayetteville, AR, 72704, USA. fgcarbon@uark.edu.
Dig Dis Sci ; 62(12): 3370-3384, 2017 12.
Article em En | MEDLINE | ID: mdl-28965192
BACKGROUND: Diet is suggested to participate in the etiology of inflammatory bowel diseases (IBD). Repeated exposure to Maillard reaction products (MRPs), molecules resulting from reduction reactions between amino acids and sugars during food heating, has been reported to be either potentially detrimental or beneficial to health. AIMS: The aim of this study is to determine the effect of repeated oral ingestion of N ε-carboxymethyllysine (CML), an advanced MRP, on the onset of two models of experimental IBD and on the gut microbiota composition of mice. METHODS: Mice received either saline (control) or N ε-carboxymethyllysine daily for 21 days. For the last week of treatment, each group was split into subgroups, receiving dextran sulfate sodium salt (DSS) or trinitrobenzenesulfonic acid (TNBS) to induce colitis. Intensity of inflammation was quantified, and cecal microbiota characterized by bacterial 16S ribosomal RNA (rRNA) amplicon sequencing. RESULTS: Daily oral administration of N ε-carboxymethyllysine did not induce intestinal inflammation and had limited impact on gut microbiota composition (Bacteroidaceae increase, Lachnospiraceae decrease). DSS and TNBS administration resulted in expected moderate experimental colitis with a shift of Bacteroidetes/Firmicutes ratio and a significant Proteobacteria increase but with distinct profiles: different Proteobacteria taxa for DSS, but mainly Enterobacteriaceae for TNBS. While N ε-carboxymethyllysine exposure failed to prevent the inflammatory response, it allowed maintenance of healthy gut microbiota profiles in mice treated with DSS (but not TNBS). CONCLUSIONS: Repeated oral exposure to CML limits dysbiosis in experimental colitis. IBD patients may modulate their microbiota profile by regulating the level and type of dietary MRP consumption.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Produtos Finais de Glicação Avançada / Colite / Disbiose / Microbioma Gastrointestinal / Lisina Tipo de estudo: Etiology_studies Limite: Animals Idioma: En Revista: Dig Dis Sci Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Produtos Finais de Glicação Avançada / Colite / Disbiose / Microbioma Gastrointestinal / Lisina Tipo de estudo: Etiology_studies Limite: Animals Idioma: En Revista: Dig Dis Sci Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Estados Unidos