Improved process conditions for increasing expression of MHC class II protein from a stable Drosophila S2 cell line.
Biotechnol Lett
; 40(1): 85-92, 2018 Jan.
Article
em En
| MEDLINE
| ID: mdl-28993910
ABSTRACT
OBJECTIVES:
To investigate the effects of operational process conditions on expression of MHC class II protein from a stable Drosophila S2 cell line.RESULTS:
When the Drosophila S2 cells were grown in vented orbitally shaken TubeSpin bioreactor 600 containers, cell growth was improved three-fold and the yield of recombinant major histocompatibility (MHC) class II protein (HLA-DR12xHis) increased four-fold over the levels observed for the same cells cultivated in roller bottles (RB) without vented caps. Culturing in RB with vented caps while increasing the rotation speed from 6 rpm to 18 rpm also improved cell growth five-fold and protein productivity three-fold which is comparable to the levels observed in the orbitally shaken containers. Protein activity was found to be almost identical between the two vessel systems tested.CONCLUSIONS:
Optimized cell culture conditions and a more efficient vessel type can enhance gas transfer and mixing and lead to substantial improvement of recombinant product yields from S2 cells.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes
/
Antígeno HLA-DR1
/
Técnicas de Cultura de Células
Limite:
Animals
Idioma:
En
Revista:
Biotechnol Lett
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Suíça