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Coding and noncoding landscape of extracellular RNA released by human glioma stem cells.
Wei, Zhiyun; Batagov, Arsen O; Schinelli, Sergio; Wang, Jintu; Wang, Yang; El Fatimy, Rachid; Rabinovsky, Rosalia; Balaj, Leonora; Chen, Clark C; Hochberg, Fred; Carter, Bob; Breakefield, Xandra O; Krichevsky, Anna M.
Afiliação
  • Wei Z; Department of Neurology, Brigham and Women's Hospital and Harvard Medical School, HMS Initiative for RNA Medicine, Boston, MA, 02115, USA.
  • Batagov AO; Vishuo Biomedical, #3-33 Teletech Park, 20 Science Park Road, Singapore, 117674, Singapore.
  • Schinelli S; Department of Drug Sciences, University of Pavia, Pavia, 27100, Italy.
  • Wang J; Beijing Genomics Institute, Shenzhen, 518083, China.
  • Wang Y; Department of Neurology, Brigham and Women's Hospital and Harvard Medical School, HMS Initiative for RNA Medicine, Boston, MA, 02115, USA.
  • El Fatimy R; Department of Neurology, Brigham and Women's Hospital and Harvard Medical School, HMS Initiative for RNA Medicine, Boston, MA, 02115, USA.
  • Rabinovsky R; Department of Neurology, Brigham and Women's Hospital and Harvard Medical School, HMS Initiative for RNA Medicine, Boston, MA, 02115, USA.
  • Balaj L; Department of Neurology and Radiology, Massachusetts General Hospital and Program in Neuroscience, Harvard Medical School, Charlestown, MA, 02129, USA.
  • Chen CC; Neurosurgery Department, University of Minnesota, Minneapolis, MN, 55455, USA.
  • Hochberg F; Department of Neurosurgery, University of California, La Jolla, San Diego, CA, 92093, USA.
  • Carter B; Scintillon Institute, San Diego, CA, 92121, USA.
  • Breakefield XO; Department of Neurosurgery, University of California, La Jolla, San Diego, CA, 92093, USA.
  • Krichevsky AM; Department of Neurology and Radiology, Massachusetts General Hospital and Program in Neuroscience, Harvard Medical School, Charlestown, MA, 02129, USA.
Nat Commun ; 8(1): 1145, 2017 10 26.
Article em En | MEDLINE | ID: mdl-29074968
ABSTRACT
Tumor-released RNA may mediate intercellular communication and serve as biomarkers. Here we develop a protocol enabling quantitative, minimally biased analysis of extracellular RNAs (exRNAs) associated with microvesicles, exosomes (collectively called EVs), and ribonucleoproteins (RNPs). The exRNA complexes isolated from patient-derived glioma stem-like cultures exhibit distinct compositions, with microvesicles most closely reflecting cellular transcriptome. exRNA is enriched in small ncRNAs, such as miRNAs in exosomes, and precisely processed tRNA and Y RNA fragments in EVs and exRNPs. EV-enclosed mRNAs are mostly fragmented, and UTRs enriched; nevertheless, some full-length mRNAs are present. Overall, there is less than one copy of non-rRNA per EV. Our results suggest that massive EV/exRNA uptake would be required to ensure functional impact of transferred RNA on brain recipient cells and predict the most impactful miRNAs in such conditions. This study also provides a catalog of diverse exRNAs useful for biomarker discovery and validates its feasibility on cerebrospinal fluid.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco Neoplásicas / RNA Mensageiro / RNA Neoplásico / RNA não Traduzido / Vesículas Extracelulares Limite: Animals / Humans Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco Neoplásicas / RNA Mensageiro / RNA Neoplásico / RNA não Traduzido / Vesículas Extracelulares Limite: Animals / Humans Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Estados Unidos