Apoptotic Effect of Nigella sativa on Human Lymphoma U937 Cells.
Pharmacogn Mag
; 13(Suppl 3): S628-S632, 2017 Oct.
Article
em En
| MEDLINE
| ID: mdl-29142424
ABSTRACT
OBJECTIVE:
Nigella sativa is from botanical Ranunculaceae family and commonly known as black seed. Apoptotic effect of N. sativa and its apoptotic signaling pathways on U937 lymphoma cells are unknown. MATERIALS ANDMETHODS:
In this study, we investigated selective cytotoxic and apoptotic effects of N. sativa extract and its apoptotic mechanisms on U937 cells. In addition, we also studied selective cytotoxic activity of thymoquinone that is the most active essential oil of N. sativa.RESULTS:
Our results showed that N. sativa extract has selective cytotoxicity and apoptotic effects on U937 cells but not ECV304 control cells. However, thymoquinone had no significant cytotoxicity against on both cells. N. sativa extract increased significantly caspase-3, BAD, and p53 gene expressions in U937 cells.CONCLUSIONS:
N. sativa may have anticancer drug potential and trigger p53-induced apoptosis in U937 lymphoma cells.SUMMARY:
This is the first study showing the apoptotic effect of Nigella sativa extract on U937 cells. Abbreviations used CI Cytotoxicity index, DMEM Dulbecco's Modified Eagle Medium, HL Hodgkin's lymphoma, MTT 3-(4,5-dimethy lthiazol-2yl)-2,5-diphenyl tetrazolium bromide, RPMI Roswell Park Memorial Institute medium.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Pharmacogn Mag
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Turquia