Induced circularly polarized luminescence arising from anion or protein binding to racemic emissive lanthanide complexes.
Methods Appl Fluoresc
; 2(2): 024007, 2014 Apr 10.
Article
em En
| MEDLINE
| ID: mdl-29148461
ABSTRACT
A circularly polarized luminescence (CPL) spectrometer has been built and used to study the binding interaction of lactate and four different proteins with racemic EuIII and TbIII complexes in aqueous solution. Lactate binding gives rise to strong induced CPL spectra, and the observed emission dissymmetry factors vary linearly with enantiomeric composition. Particularly strong induced TbIII CPL also characterizes the binding interaction of alpha-1-acid glycoprotein with a dissociation constant, Kd, of 2.5 µM.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Methods Appl Fluoresc
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Reino Unido