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Large and Small Assembly: Combining Functional Macromolecules with Small Peptides to Control the Morphology of Skeletal Muscle Progenitor Cells.
Li, Rui; McRae, Natasha L; McCulloch, Daniel R; Boyd-Moss, Mitchell; Barrow, Colin J; Nisbet, David R; Stupka, Nicole; Williams, Richard J.
Afiliação
  • Li R; Centre for Chemistry and Biotechnology , Deakin University , Waurn Ponds 3216 , Australia.
  • McRae NL; Coconut Research Institute , Chinese Academy of Tropical Agricultural Sciences , Wenchang , Hainan 571339 , China.
  • McCulloch DR; School of Medicine, Centre for Molecular and Medical Research SRC , Deakin University , Waurn Ponds 3216 , Australia.
  • Boyd-Moss M; School of Medicine, Centre for Molecular and Medical Research SRC , Deakin University , Waurn Ponds 3216 , Australia.
  • Barrow CJ; Biofab3D , St. Vincent's Hospital , Fitzroy 3065 , Australia.
  • Nisbet DR; School of Engineering , RMIT University , Bundoora 3083 , Australia.
  • Stupka N; Centre for Chemistry and Biotechnology , Deakin University , Waurn Ponds 3216 , Australia.
  • Williams RJ; Research School of Engineering , The Australian National University , Canberra 2601 , Australia.
Biomacromolecules ; 19(3): 825-837, 2018 03 12.
Article em En | MEDLINE | ID: mdl-29389119
ABSTRACT
The material properties of natural tissues, such as skeletal muscle, are highly sophisticated and are synthetically challenging to mimic. Using natural biomacromolecules to functionalize self-assembled peptide (SAP) hydrogels has the potential to increase the utility of these materials by more closely reproducing the natural cellular environment. Here, to demonstrate that a conserved co-assembly pathway can retain distinct function, the biocompatible peptide derivative Fmoc-FRGDF was co-assembled with either a sulfated polysaccharide, fucoidan, or the provisional matrix proteoglycan, versican. Our results demonstrate that thermodynamically driven co-assembly with biologically active macromolecules is facile, stable, and does not affect the final assembled nanostructure. Biologically, the incorporation of these functionally distinct molecules had no effect on C2C12 myoblast proliferation and viability but strongly altered their morphology. The surface area of myoblasts cultured on the fucoidan scaffold was reduced at 24 and 72 h post seeding, with a reduction in the formation of multinucleated syncytia. Myoblasts cultured on versican scaffolds were smaller compared to cells grown on the empty vector scaffolds at 24 h but not 72 h post seeding, with multinucleated syncytia formation being unaffected. This work allows programmed and distinct morphological effects of cell behavior, paving the way for further mechanistic studies.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos / Polissacarídeos / Mioblastos Esqueléticos / Proliferação de Células / Nanoestruturas / Versicanas / Alicerces Teciduais Limite: Humans Idioma: En Revista: Biomacromolecules Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Austrália

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos / Polissacarídeos / Mioblastos Esqueléticos / Proliferação de Células / Nanoestruturas / Versicanas / Alicerces Teciduais Limite: Humans Idioma: En Revista: Biomacromolecules Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Austrália