[Rapid determination of 25 drug residues in aquatic products by ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry].
Se Pu
; 36(2): 114-124, 2018 Feb 08.
Article
em Zh
| MEDLINE
| ID: mdl-29582597
ABSTRACT
An ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry (UPLC-Q Orbitrap HRMS) method was developed for the rapid screening of 25 drug residues in aquatic products by the application of a novel enhanced matrix removal of lipids (EMR-Lipid). The samples were extracted with acetonitrile, cleaned-up with EMR-Lipid, and salted out with 3.0 g sodium chloride and 3.0 g sodium sulfate. The separation of the 25 drug residues was performed on an ACQUITY UPLC BEH C18 column (100 mm×2.1 mm, 1.7 µm) with gradient elution. Acetonitrile containing 0.1% (v/v) formic acid and 0.1% (v/v) formic acid aqueous solution were used as mobile phases. The compounds were detected by Full scan/date dependent MS/MS (Full MS/dd-MS2) via heated electrospray ionization (HESI) source. The calibration curves were linear with correlation coefficients (r) greater than 0.997. The limits of detection (LODs) ranged from 0.1 µg/kg to 1.0 µg/kg. The average spiked recoveries of the 25 target compounds were between 70.1% and 108.9% with relative standard derivations (RSDs) from 2.1% to 13.8%. Compared with the previous methods, this method has characteristics of simpler sample preparation and higher sensitivity.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas
/
Resíduos de Drogas
/
Cromatografia Líquida de Alta Pressão
/
Alimentos Marinhos
Idioma:
Zh
Revista:
Se Pu
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China