Anoctamin 9/TMEM16J is a cation channel activated by cAMP/PKA signal.
Cell Calcium
; 71: 75-85, 2018 05.
Article
em En
| MEDLINE
| ID: mdl-29604966
ABSTRACT
Anoctamins (ANOs) are multifunctional membrane proteins that consist of 10 homologs. ANO1 (TMEM16A) and ANO2 (TMEM16B) are anion channels activated by intracellular calcium that meditate numerous physiological functions. ANO6 is a scramblase that redistributes phospholipids across the cell membrane. The other homologs are not well characterized. We found ANO9/TMEM16J is a cation channel activated by a cAMP-dependent protein kinase A (PKA). Intracellular cAMP-activated robust currents in whole cells expressing ANO9, which were inhibited by a PKA blocker. A cholera toxin that persistently stimulated adenylate cyclase activated ANO9 as did the application of PKA. The cAMP-induced ANO9 currents were permeable to cations. The cAMP-dependent ANO9 currents were augmented by intracellular Ca2+. Ano9 transcripts were predominant in the intestines. Human intestinal SW480 cells expressed high levels of Ano9 transcripts and showed PKA inhibitor-reversible cAMP-dependent currents. We conclude that ANO9 is a cation channel activated by a cAMP/PKA pathway and could play a role in intestine function.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transdução de Sinais
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Ativação do Canal Iônico
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Proteínas Quinases Dependentes de AMP Cíclico
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AMP Cíclico
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Proteínas de Transferência de Fosfolipídeos
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Anoctaminas
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Proteínas de Membrana
Limite:
Animals
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Humans
Idioma:
En
Revista:
Cell Calcium
Ano de publicação:
2018
Tipo de documento:
Article