Mechanical strain induced phospho-proteomic signaling in uterine smooth muscle cells.
J Biomech
; 73: 99-107, 2018 05 17.
Article
em En
| MEDLINE
| ID: mdl-29661501
ABSTRACT
Mechanical strain associated with the expanding uterus correlates with increased preterm birth rates. Mechanical signals result in a cascading network of protein phosphorylation events. These signals direct cellular activities and may lead to changes in contractile phenotype and calcium signaling. In this study, the complete phospho-proteome of uterine smooth muscle cells subjected to mechanical strain for 5â¯min was compared to un-strained controls. Statistically significant, differential phosphorylation events were annotated by Ingenuity Pathway Analysis to elucidate mechanically induced phosphorylation networks. Mechanical strain leads to the direct activation of ERK1/2, HSPB1, and MYL9, in addition to phosphorylation of PAK2, vimentin, DOCK1, PPP1R12A, and PTPN11 at previously unannotated sites. These results suggest a novel network reaction to mechanical strain and reveal proteins that participate in the activation of contractile mechanisms leading to preterm labor.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfoproteínas
/
Estresse Mecânico
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Útero
/
Miócitos de Músculo Liso
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Proteômica
Limite:
Adult
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Animals
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Female
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Humans
Idioma:
En
Revista:
J Biomech
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Estados Unidos