Capillary ultra performance liquid chromatography-tandem mass spectrometry analysis of tienilic acid metabolites in urine following intravenous administration to the rat.
J Chromatogr B Analyt Technol Biomed Life Sci
; 1087-1088: 142-148, 2018 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-29738964
ABSTRACT
Capillary scale (100â¯mmâ¯×â¯150⯵m id) UPLC/MS/MS, performed using reversed-phase gradient chromatography on sub 2⯵m particles, has been successfully employed for the characterization of the metabolites of the drug tienilic acid (TA) excreted via the urine following oral administration to the rat. The capillary LC system provided a significant increase (range ca. 11-33-fold) in sensitivity compared with a conventional 150â¯mmâ¯×â¯2.1â¯mm id UPLC system. An investigation of the effect of the injection volume and sample mass loading on the capillary column on the results obtained for both endogenous metabolites and TA was performed. This demonstrated that the injection of up to 2⯵L of rat urine onto the system was permitted whilst still providing excellent chromatographic results and robustness. Qualitative analysis of the urine revealed the presence of TA itself and a total of 15 metabolites of the drug, including those resulting from biotransformations such as hydroxylation or conjugation. The capillary chromatography system was shown to be robust, and capable of providing comprehensive drug metabolite profiles from small format urine samples such as those obtained from preclinical studies in rodents.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ticrinafeno
/
Cromatografia Líquida de Alta Pressão
/
Espectrometria de Massas em Tandem
Tipo de estudo:
Qualitative_research
Limite:
Animals
Idioma:
En
Revista:
J Chromatogr B Analyt Technol Biomed Life Sci
Assunto da revista:
ENGENHARIA BIOMEDICA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Estados Unidos