Process optimization for the rapid production of adenoviral vectors for clinical trials in a disposable bioreactor system.

Chen, Ke-Da; Wu, Xiao-Xin; Yu, Dong-Shan; Ou, Hui-Lin; Li, Yan-Hua; Zhou, Yu-Qing; Li, Lan-Juan

Chen, Ke-Da; Wu, Xiao-Xin; Yu, Dong-Shan; Ou, Hui-Lin; Li, Yan-Hua; Zhou, Yu-Qing; Li, Lan-Juan.

Afiliação

Chen KD; State Key Laboratory for Diagnosis and Treatment of Infectious Disease, Collaborative Innovative Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China.
Wu XX; State Key Laboratory for Diagnosis and Treatment of Infectious Disease, Collaborative Innovative Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China.
Yu DS; State Key Laboratory for Diagnosis and Treatment of Infectious Disease, Collaborative Innovative Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China.
Ou HL; State Key Laboratory for Diagnosis and Treatment of Infectious Disease, Collaborative Innovative Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China.
Li YH; State Key Laboratory for Diagnosis and Treatment of Infectious Disease, Collaborative Innovative Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China.
Zhou YQ; State Key Laboratory for Diagnosis and Treatment of Infectious Disease, Collaborative Innovative Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China.
Li LJ; State Key Laboratory for Diagnosis and Treatment of Infectious Disease, Collaborative Innovative Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China. ljli@zju.edu.cn.

Appl Microbiol Biotechnol ; 102(15): 6469-6477, 2018 Aug.

Article em En | MEDLINE | ID: mdl-29858958

Assuntos

Adenoviridae/fisiologia; Reatores Biológicos; Técnicas de Cultura de Células/instrumentação; Terapia Genética/instrumentação; Vetores Genéticos/fisiologia; Microbiologia Industrial/instrumentação; Microbiologia Industrial/métodos; Células HEK293; Humanos

Palavras-chave

Adenoviral vector; Disposable bioreactor; HEK293 cells; Process optimization

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Microbiologia Industrial / Terapia Genética / Adenoviridae / Técnicas de Cultura de Células / Reatores Biológicos / Vetores Genéticos Tipo de estudo: Guideline Limite: Humans Idioma: En Revista: Appl Microbiol Biotechnol Ano de publicação: 2018 Tipo de documento: Article País de afiliação: China

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