Mushroom extract inhibits ultraviolet B-induced cellular senescence in human keratinocytes.
Cytotechnology
; 70(3): 1001-1008, 2018 Jun.
Article
em En
| MEDLINE
| ID: mdl-29860669
ABSTRACT
Mushrooms possess various bioactivities and are used as nutritional supplements and medicinal products. Twenty-nine bioactive components have been extracted recently from mushrooms grown in Nepal. In this study, we evaluated the ability of these mushroom extracts to augment SIRT1, a mammalian SIR2 homologue localized in cytosol and nuclei. We established a system for screening food ingredients that augment the SIRT1 promoter in HaCaT cells, and identified a SIRT1-augmenting mushroom extract (number 28, Trametes versicolor). UVB irradiation induced cellular senescence in HaCaT cells, as evidenced by increased activity and expression of cellular senescence markers including senescence-associated ß-galactosidase, p21, p16, phosphorylated p38, and γH2AX. Results clearly showed that the mushroom extract (No. 28) suppressed the ultraviolet B irradiation-induced cellular senescence in HaCaT cells possibly through augmenting SIRT1 expression.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Cytotechnology
Assunto da revista:
BIOTECNOLOGIA
/
GENETICA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Japão