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Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing.
Bossé, Janine T; Li, Yanwen; Fernandez Crespo, Roberto; Lacouture, Sonia; Gottschalk, Marcelo; Sárközi, Rita; Fodor, László; Casas Amoribieta, Maria; Angen, Øystein; Nedbalcova, Katerina; Holden, Matthew T G; Maskell, Duncan J; Tucker, Alexander W; Wren, Brendan W; Rycroft, Andrew N; Langford, Paul R.
Afiliação
  • Bossé JT; Section of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, UK. Electronic address: j.bosse@imperial.ac.uk.
  • Li Y; Section of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, UK.
  • Fernandez Crespo R; Section of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, UK.
  • Lacouture S; Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, Québec, Canada.
  • Gottschalk M; Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, Québec, Canada.
  • Sárközi R; Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, Budapest, Hungary.
  • Fodor L; Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, Budapest, Hungary.
  • Casas Amoribieta M; OVISLAB S.L., Barcelona, Spain.
  • Angen Ø; Department of Microbiology and Infection Control, Statens Serum Institut, Copenhagen, Denmark.
  • Nedbalcova K; Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic.
  • Holden MTG; The Wellcome Trust Sanger Institute, Hinxton, UK.
  • Maskell DJ; Department of Veterinary Medicine, University of Cambridge, Cambridge, UK.
  • Tucker AW; Department of Veterinary Medicine, University of Cambridge, Cambridge, UK.
  • Wren BW; Faculty of Infectious & Tropical Diseases, London School of Hygiene & Tropical Medicine, London, UK.
  • Rycroft AN; Department of Pathology and Pathogen Biology, The Royal Veterinary College, Hawkshead Campus, UK.
  • Langford PR; Section of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, UK.
Vet Microbiol ; 220: 83-89, 2018 Jul.
Article em En | MEDLINE | ID: mdl-29885806
ABSTRACT
Problems with serological cross-reactivity have led to development of a number of PCRs (individual and multiplex) for molecular typing of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. Most of these assays were developed for detection of specific amplicons within capsule biosynthetic genes before the availability of complete sequences for the different serovars. Here we describe comparative analysis of the complete capsular loci for all 18 serovars of A. pleuropneumoniae, and development of two multiplex PCRs for comprehensive capsule typing of this important pig pathogen.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polissacarídeos Bacterianos / Doenças dos Suínos / Cápsulas Bacterianas / Actinobacillus pleuropneumoniae / Análise de Sequência / Reação em Cadeia da Polimerase Multiplex Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Vet Microbiol Ano de publicação: 2018 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polissacarídeos Bacterianos / Doenças dos Suínos / Cápsulas Bacterianas / Actinobacillus pleuropneumoniae / Análise de Sequência / Reação em Cadeia da Polimerase Multiplex Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Vet Microbiol Ano de publicação: 2018 Tipo de documento: Article