Normalization of disrupted clock gene expression in males with tetraplegia: a crossover randomized placebo-controlled trial of melatonin supplementation.
Spinal Cord
; 56(11): 1076-1083, 2018 Nov.
Article
em En
| MEDLINE
| ID: mdl-29985456
ABSTRACT
STUDY DESIGN:
Crossover double blind, randomized placebo-controlled trial.OBJECTIVES:
Circadian oscillators are located both in the brain and in peripheral organs. Melatonin, the main brain-derived hormone governing circadian variations, is highly associated with daylight patterns. However, in subjects with tetraplegia the melatonin levels are blunted. Here we studied peripheral oscillators in peripheral blood mononuclear cells (PBMCs) in males with tetraplegia by examining how exogenous melatonin may influence the expression of clock gene mRNAs.SETTING:
Sunnaas Rehabilitation Hospital, Nesoddtangen, Norway.METHODS:
Six males with tetraplegia received 2 mg of melatonin or placebo 4 days before the study period. We also included six able-bodied men sleeping or kept awake during the night. Plasma samples were collected four times during a 24-h period. The mRNA expression levels of the clock genes PER1, PER2, BMAL1, and REV-ERBα were quantified in PBMCs using quantitative RT-PCR.RESULTS:
The mRNA expression levels of PER-1 and -2 and REV-ERBα were increased at 0400 h compared with the able-bodied controls (p < 0.05). Melatonin supplementation changed mRNA peak-time toward the time of supplementation.CONCLUSIONS:
Several peripheral clock genes displayed distorted expression levels in tetraplegia. Supplementation with melatonin changed the mRNA expression levels of these genes toward those observed among able-bodied. SPONSORSHIP Financial support was provided from the Throne Holst Foundation, Sunnaas Rehabilitation hospital and the University of Ferrara (FAR2016).
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Quadriplegia
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Fármacos do Sistema Nervoso Central
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Proteínas CLOCK
/
Melatonina
Tipo de estudo:
Clinical_trials
Limite:
Adult
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Humans
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Male
/
Middle aged
Idioma:
En
Revista:
Spinal Cord
Assunto da revista:
NEUROLOGIA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Noruega