Chitosans as new tools against biofilms formation on the surface of silicone urinary catheters.
Int J Biol Macromol
; 118(Pt B): 2193-2200, 2018 Oct 15.
Article
em En
| MEDLINE
| ID: mdl-30012489
ABSTRACT
Urinary catheters contamination by microorganisms is a major cause of hospital acquired infections and represents a limitation for long-term use. In this work, biofilms of Klebsiella pneumoniae and Escherichia coli clinical isolates were developed on urinary catheters for 48 and 72â¯h in artificial urine medium (AUM) with different molecular weight chitosans (AUM-CS solutions) at pHâ¯5.0. The number of viable bacteria was determined by standard plate count agar while crystal violet (CV) staining was carried out to assess biomass production (optical density at 570â¯nm) in the mentioned conditions. Re-growth of each strain was also evaluated after 24â¯h re-incubation of the treated catheters. Significant decreases of log CFU/catheter and biomass production were observed for all the biofilms developed in AUM-CS compared with the controls in AUM. The percentages of biofilm removal were slightly higher for E. coli biofilms (up to 90.4%) than those of K. pneumoniae (89.7%); in most cases, the complete inhibition of bacterial re-growth on treated catheter pieces was observed. Contact time influenced chitosan efficacy rather than its molecular weight or the biofilms age. The results confirmed the potentiality of chitosans as a biomacromolecule tool to contrast biofilm formation and reduce bacterial re-growth on urinary catheters.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Silicones
/
Biofilmes
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Quitosana
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Cateteres Urinários
Idioma:
En
Revista:
Int J Biol Macromol
Ano de publicação:
2018
Tipo de documento:
Article