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Evolution shapes the responsiveness of the D-box enhancer element to light and reactive oxygen species in vertebrates.
Pagano, Cristina; Siauciunaite, Rima; Idda, Maria L; Ruggiero, Gennaro; Ceinos, Rosa M; Pagano, Martina; Frigato, Elena; Bertolucci, Cristiano; Foulkes, Nicholas S; Vallone, Daniela.
Afiliação
  • Pagano C; Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany.
  • Siauciunaite R; CNR, ISASI "E. Caianiello" Pozzuoli, Naples, Italy.
  • Idda ML; Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany.
  • Ruggiero G; Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany.
  • Ceinos RM; Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland, USA.
  • Pagano M; Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany.
  • Frigato E; Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany.
  • Bertolucci C; Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy.
  • Foulkes NS; Facultade de Bioloxía, Universidade de Vigo, Vigo, Spain.
  • Vallone D; Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany.
Sci Rep ; 8(1): 13180, 2018 09 04.
Article em En | MEDLINE | ID: mdl-30181539
The circadian clock is a highly conserved cell-autonomous mechanism that directs daily rhythms in most aspects of biology. Daily entrainment by environmental signals, notably light, is essential for its function. However, our understanding of the mechanisms and the evolution of photic entrainment remains incomplete. Fish represent attractive models for exploring how light regulates the circadian clock due to the direct light sensitivity of their peripheral clocks. Central to this property is the light induced expression of clock genes that is mediated by D-box enhancer elements. Here, using zebrafish cells, we reveal that the light responsive D-box enhancer serves as a nuclear target for reactive oxygen species (ROS). We demonstrate that exposure to short wavelengths of visible light triggers increases in ROS levels via NADPH oxidase activity. Elevated ROS activates the JNK and p38 MAP kinases and in turn, induces clock gene expression via the D-box. In blind cavefish and mammals, where peripheral clocks are no longer entrained by direct illumination, ROS levels are still increased upon light exposure. However, in these species ROS no longer induces D-box driven clock gene transcription. Thus, during evolution, alterations in ROS-responsive signal transduction pathways underlie fundamental changes in peripheral clock photoentrainment.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cyprinidae / Elementos Facilitadores Genéticos / Espécies Reativas de Oxigênio / Relógios Circadianos Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Alemanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cyprinidae / Elementos Facilitadores Genéticos / Espécies Reativas de Oxigênio / Relógios Circadianos Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Alemanha