Circular RNA hsa_circ_0072995 promotes breast cancer cell migration and invasion through sponge for miR-30c-2-3p.
Epigenomics
; 10(9): 1229-1242, 2018 09.
Article
em En
| MEDLINE
| ID: mdl-30182731
ABSTRACT
AIM:
To study the role of hsa_circ_0072995 in regulating the invasion and migration of breast cancer cells. MATERIALS &METHODS:
Hsa_circ_0072995 expression was confirmed by quantitative real-time PCR; evaluating the migration and invasion of breast cancer cells through transwell assay; predicating circRNA/microRNAs interaction using the miRanda and RNAhybrid software; identifying the relationship between hsa_circ_0072995 and miR-30c-2-3p by luciferase activity assay; detecting the location of hsa_circ_0072995 by Fluorescence in situ hybridization assay.RESULTS:
Hsa_circ_0072995 was significantly upregulated in MDA-MB-231 cells compared with MCF-7 cells. Hsa_circ_0072995 regulated the invasion and migration of breast cancer cells. Hsa_circ_0072995 existed in the nucleus and cytoplasm, and the proportion of the two was roughly equal. Hsa_circ_0072995 bound to miR-30c-2-3p. Overexpression of miR-30c-2-3p inhibited breast cancer cells migration and invasion. Low expression of miR-30c-2-3p was correlated with poor overall survival by The Cancer Genome Atlas database.CONCLUSION:
Hsa_circ_0072995 may be a novel biomarker for breast cancer, and may function in metastasis of breast cancer.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Mama
/
RNA
/
Biomarcadores Tumorais
/
Movimento Celular
/
MicroRNAs
Limite:
Female
/
Humans
Idioma:
En
Revista:
Epigenomics
Ano de publicação:
2018
Tipo de documento:
Article