Microbe-mediate transformation of echinocystic acid by whole cells of filamentous fungus Cunninghamella blakesleana CGMCC 3.910.

Fu, Shaobin; Feng, Xu; Sun, Di-An

Fu, Shaobin; Feng, Xu; Sun, Di-An.

Afiliação

Fu S; Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, China. fushb@126.com.
Feng X; Department of Pharmacy, Zunyi Medical University, Zunyi, 563000, China. fushb@126.com.
Sun DA; Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, China.

Mol Biol Rep ; 45(6): 2795-2800, 2018 Dec.

Article em En | MEDLINE | ID: mdl-30194559

ABSTRACT

ABSTRACT

Structural modification of echinocystic acid (EA), a pentacyclic triterpenoid with wide spread biological activities was investigated by microbial transformation. Microbe-mediate transformation of EA was carried out by filamentous fungus Cunninghamella blakesleana CGMCC 3.910. Four metabolites 3ß, 7ß, 16α-trihydroxy-olean-12-en-28-oic acid (EA-2); 3ß, 7ß, 16ß,19ß-tetrahydroxy-olean-12-en-28-oic acid (EA-3); 3ß, 7ß, 16α, 21ß-tetrahydroxy-olean-12-en-28-oic acid (EA-4); 3ß, 7ß, 16α-trihydroxy-olean-11, 13(18)-dien-28-oic acid (EA-5) were produced. Structures of transformed products were elucidated by 1D and 2D NMR and HR-MS data. EA-3 and EA-4 were new compounds.

Assuntos

Cunninghamella/genética; Ácido Oleanólico/análogos & derivados; Biotransformação/genética; Estrutura Molecular; Ácido Oleanólico/genética; Ácido Oleanólico/metabolismo; Triterpenos Pentacíclicos; Triterpenos

Palavras-chave

Biocatalysis; Echinocystic acid; Fungus; Pentacyclic triterpenoids

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ácido Oleanólico / Cunninghamella Idioma: En Revista: Mol Biol Rep Ano de publicação: 2018 Tipo de documento: Article País de afiliação: China

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