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Genomic profiles of primary and metastatic esophageal adenocarcinoma identified via digital sorting of pure cell populations: results from a case report.
Isidori, Federica; Malvi, Deborah; Fittipaldi, Silvia; Forcato, Claudio; Bozzarelli, Isotta; Sala, Claudia; Raulli, Giovanni; D'Errico, Antonia; Fiorentino, Michelangelo; Seri, Marco; Krishnadath, Kausilia K; Bonora, Elena; Mattioli, Sandro.
Afiliação
  • Isidori F; PhD program in Cardio-Nephro-Thoracic Sciences, University of Bologna, Bologna, Italy.
  • Malvi D; Unit of Medical Genetics, Department of Medical and Surgical Sciences (DIMEC), University of Bologna, Policlinico St. Orsola-Malpighi Hospital, Bologna, Italy.
  • Fittipaldi S; Department of Experimental, Diagnostic and Specialty Medicine (DIMES), Institute of Oncology and Transplant Pathology, University of Bologna, Policlinico St. Orsola-Malpighi Hospital, Bologna, Italy.
  • Forcato C; Department of Experimental, Diagnostic and Specialty Medicine (DIMES), Institute of Oncology and Transplant Pathology, University of Bologna, Policlinico St. Orsola-Malpighi Hospital, Bologna, Italy.
  • Bozzarelli I; Menarini Silicon Biosystems, Bologna, Italy.
  • Sala C; PhD program in Cardio-Nephro-Thoracic Sciences, University of Bologna, Bologna, Italy.
  • Raulli G; Unit of Medical Genetics, Department of Medical and Surgical Sciences (DIMEC), University of Bologna, Policlinico St. Orsola-Malpighi Hospital, Bologna, Italy.
  • D'Errico A; Department of Physics and Astronomy, University of Bologna, Bologna, Italy.
  • Fiorentino M; Unit of Pathology - AUSL Romagna, Ravenna, Italy.
  • Seri M; Department of Experimental, Diagnostic and Specialty Medicine (DIMES), Institute of Oncology and Transplant Pathology, University of Bologna, Policlinico St. Orsola-Malpighi Hospital, Bologna, Italy.
  • Krishnadath KK; Department of Experimental, Diagnostic and Specialty Medicine (DIMES), Institute of Oncology and Transplant Pathology, University of Bologna, Policlinico St. Orsola-Malpighi Hospital, Bologna, Italy.
  • Bonora E; Unit of Medical Genetics, Department of Medical and Surgical Sciences (DIMEC), University of Bologna, Policlinico St. Orsola-Malpighi Hospital, Bologna, Italy.
  • Mattioli S; Center for Experimental and Molecular Medicine, Academic Medical Center, Department of Gastroenterology and Hepatology, Amsterdam, The Netherlands.
BMC Cancer ; 18(1): 889, 2018 Sep 12.
Article em En | MEDLINE | ID: mdl-30208867
BACKGROUND: We report on a female patient who underwent primary radical resection for a stage 2B Her-2-positive Barrett's-type esophageal adenocarcinoma (EAC). Despite Her-2 targeted therapy, her disease recurred and required repeated metastectomies. CASE PRESENTATION: Digital cell sorting and targeted sequencing of cancer sub-clones from EAC and metastases revealed a completely mutated TP53, whereas the sorted stromal cells were wild-type. Her-2 amplification was significantly lower in the metastases when the patient became therapy-resistant. CONCLUSIONS: The mechanism of therapy resistance illustrated by this case could only be detected through accurate analysis of tumor sub-populations. Investigating tumor sub-populations of recurrent disease is important for adjusting therapy in recurrent EAC.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Esofágicas / Adenocarcinoma / Genômica / Evolução Clonal / Sequenciamento Completo do Genoma Tipo de estudo: Prognostic_studies Limite: Female / Humans Idioma: En Revista: BMC Cancer Assunto da revista: NEOPLASIAS Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Itália

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Esofágicas / Adenocarcinoma / Genômica / Evolução Clonal / Sequenciamento Completo do Genoma Tipo de estudo: Prognostic_studies Limite: Female / Humans Idioma: En Revista: BMC Cancer Assunto da revista: NEOPLASIAS Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Itália