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[Optimization of ion selective electrode method in detection of urinary fluorine].
Zhang, F G; Yan, H F; Zhao, W.
Afiliação
  • Zhang FG; National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Article em Zh | MEDLINE | ID: mdl-30248752
ABSTRACT

Objective:

To optimize the ion selective electrode method of measuring urinary fluorine in WS/T 30-1996.

Methods:

The volume of 5 mol/L NaOH solution was fixed to confect the TISAB buffer solution. The samples were prepared by mixing 5 ml urine and equal volume of TISAB solution. Fluorine electrode and calomel electrode was used to test. When the potential value changed less than ±0.5 mV in one minute, it could be recorded. The standard curve method was selected as a quantitive method.

Results:

The linear range of this method was 0.07~50.0 mg/L, E=238.96-57.50lgC, r=0.999 8. The LOD and LOQ were 0.02 mg/L and 0.07 mg/L (with 5 ml urine) , respectively. The RSD of intra -and inter-batch precision were 0.80%~2.82% and 2.17%~2.86%, respectively. The recovery rate was 102%~107%. The urine sample could be preserved stably for 14 days at room temperature, and for 30 days at 4 ℃ and -20 ℃.

Conclusion:

In this method, the preparation process of TISAB buffer solution was simplified, and the linear range was expanded. It could meet the needs of occupational population detection.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Eletrodos Seletivos de Íons / Flúor Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: Zh Revista: Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi Assunto da revista: MEDICINA OCUPACIONAL Ano de publicação: 2018 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Eletrodos Seletivos de Íons / Flúor Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: Zh Revista: Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi Assunto da revista: MEDICINA OCUPACIONAL Ano de publicação: 2018 Tipo de documento: Article País de afiliação: China