[Optimization of ion selective electrode method in detection of urinary fluorine].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
; 36(6): 466-468, 2018 Jun 20.
Article
em Zh
| MEDLINE
| ID: mdl-30248752
ABSTRACT
Objective:
To optimize the ion selective electrode method of measuring urinary fluorine in WS/T 30-1996.Methods:
The volume of 5 mol/L NaOH solution was fixed to confect the TISAB buffer solution. The samples were prepared by mixing 5 ml urine and equal volume of TISAB solution. Fluorine electrode and calomel electrode was used to test. When the potential value changed less than ±0.5 mV in one minute, it could be recorded. The standard curve method was selected as a quantitive method.Results:
The linear range of this method was 0.07~50.0 mg/L, E=238.96-57.50lgC, r=0.999 8. The LOD and LOQ were 0.02 mg/L and 0.07 mg/L (with 5 ml urine) , respectively. The RSD of intra -and inter-batch precision were 0.80%~2.82% and 2.17%~2.86%, respectively. The recovery rate was 102%~107%. The urine sample could be preserved stably for 14 days at room temperature, and for 30 days at 4 â and -20 â.Conclusion:
In this method, the preparation process of TISAB buffer solution was simplified, and the linear range was expanded. It could meet the needs of occupational population detection.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Eletrodos Seletivos de Íons
/
Flúor
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
Zh
Revista:
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
Assunto da revista:
MEDICINA OCUPACIONAL
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China