[Effectiveness of incorporation of spermine-condensed viral DNA into liposomes. Interaction of liposomes with cells]. / Effektivnost' vkliucheniia kondensirovannykh sperminon virusnykh DNK v liposomy. Vzaimodeistvie liposom s kletkami.

ABSTRACT

DNA from bacteriophage lambda or monkey adenovirus type 7 have been condensed with spermine and included into three types of liposomes ethereal, obtained by inverted phases technique and by Ca++ fusion. Compact DNA form presents a tor with 100 nm external diameter and 430 nm width. It can be included into 100 nm or larger liposomes. Total preparation contains 15-18% of the required liposomes. Liposomal fractions with included DNA were separated from empty liposomes by step gradient of ficoll 400. Liposomal fraction having included DNA contains 15-18% of common lipid. Liposomal interaction with monkey cell cultures has been studied.