Multiple domains for the chicken cellular sequences homologous to the v-ets oncogene of the E26 retrovirus.
Mol Cell Biol
; 7(2): 806-12, 1987 Feb.
Article
em En
| MEDLINE
| ID: mdl-3029569
ABSTRACT
We have investigated the structure of chicken genomic DNA homologous to v-ets, the second cell-derived oncogene of avian retrovirus E26. We isolated a c-ets locus spanning ca. 30.0 kilobase pairs (kbp) in the chicken genome with homologies to 1,202 nucleotides (nt) of v-ets (total length, 1,508 nt) distributed in six clusters along 18.0 kbp of the cloned DNA. The 5'-distal part of v-ets (224 nt) was homologous to chicken cellular sequences contained upstream within a single 16.0-kbp EcoRI fragment as two typical exons but not found transcribed into the major 7.5-kb c-ets (or 4.0-kb c-myb) RNA species. Between these two v-ets-related cellular sequences we found ca 40.0 kbp of v-ets-unrelated DNA. Finally, the most 3' region of homology to v-ets in the cloned DNA was shown to consist of a truncated exon lacking the nucleotides coding for the 16 carboxy-terminal amino acids of the viral protein but colinear to one of the two human c-ets loci, c-ets-2.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oncogenes
/
Proto-Oncogenes
/
Galinhas
/
Vírus da Leucose Aviária
Limite:
Animals
Idioma:
En
Revista:
Mol Cell Biol
Ano de publicação:
1987
Tipo de documento:
Article