Recombinant infectious bronchitis viruses expressing heterologous S1 subunits: potential for a new generation of vaccines that replicate in Vero cells.
J Gen Virol
; 99(12): 1681-1685, 2018 12.
Article
em En
| MEDLINE
| ID: mdl-30355423
ABSTRACT
The spike glycoprotein (S) of infectious bronchitis virus (IBV) comprises two subunits, S1 and S2. We have previously demonstrated that the S2 subunit of the avirulent Beau-R strain is responsible for its extended cellular tropism for Vero cells. Two recombinant infectious bronchitis viruses (rIBVs) have been generated; the immunogenic S1 subunit is derived from the IBV vaccine strain, H120, or the virulent field strain, QX, within the genetic background of Beau-R. The rIBVs BeauR-H120(S1) and BeauR-QX(S1) are capable of replicating in primary chicken kidney cell cultures and in Vero cells. These results demonstrate that rIBVs are able to express S1 subunits from genetically diverse strains of IBV, which will enable the rational design of a future generation of IBV vaccines that may be grown in Vero cells.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças das Aves Domésticas
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Replicação Viral
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Proteínas Recombinantes
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Vacinas Virais
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Infecções por Coronavirus
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Vírus da Bronquite Infecciosa
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Glicoproteína da Espícula de Coronavírus
Limite:
Animals
Idioma:
En
Revista:
J Gen Virol
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Reino Unido