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In Vivo Extracellular pH Mapping of Tumors Using Electron Paramagnetic Resonance.
Komarov, Denis A; Ichikawa, Yuki; Yamamoto, Kumiko; Stewart, Neil J; Matsumoto, Shingo; Yasui, Hironobu; Kirilyuk, Igor A; Khramtsov, Valery V; Inanami, Osamu; Hirata, Hiroshi.
Afiliação
  • Komarov DA; Division of Bioengineering and Bioinformatics, Graduate School of Information Science and Technology , Hokkaido University , North 14, West 9 , Kita-ku, Sapporo , 060-0814 , Japan.
  • Ichikawa Y; Division of Bioengineering and Bioinformatics, Graduate School of Information Science and Technology , Hokkaido University , North 14, West 9 , Kita-ku, Sapporo , 060-0814 , Japan.
  • Yamamoto K; Laboratory of Radiation Biology, Graduate School of Veterinary Medicine , Hokkaido University , North 18, West 9 , Kita-ku, Sapporo , 060-0818 , Japan.
  • Stewart NJ; Division of Bioengineering and Bioinformatics, Graduate School of Information Science and Technology , Hokkaido University , North 14, West 9 , Kita-ku, Sapporo , 060-0814 , Japan.
  • Matsumoto S; Division of Bioengineering and Bioinformatics, Graduate School of Information Science and Technology , Hokkaido University , North 14, West 9 , Kita-ku, Sapporo , 060-0814 , Japan.
  • Yasui H; Central Institute of Isotope Science , Hokkaido University , North 15, West 7 , Kita-ku,Sapporo , 060-0815 , Japan.
  • Kirilyuk IA; N. N. Vorozhtsov Novosibirsk Institute of Organic Chemistry , 9, Ac. Lavrentieva Ave. , Novosibirsk , 630090 , Russia.
  • Khramtsov VV; Department of Biochemistry and In Vivo Multifunctional Magnetic Resonance Center , West Virginia University, Robert C. Byrd Health Sciences Center , 1 Medical Center Drive , Morgantown , West Virginia 26506 , United States.
  • Inanami O; Laboratory of Radiation Biology, Graduate School of Veterinary Medicine , Hokkaido University , North 18, West 9 , Kita-ku, Sapporo , 060-0818 , Japan.
  • Hirata H; Division of Bioengineering and Bioinformatics, Graduate School of Information Science and Technology , Hokkaido University , North 14, West 9 , Kita-ku, Sapporo , 060-0814 , Japan.
Anal Chem ; 90(23): 13938-13945, 2018 12 04.
Article em En | MEDLINE | ID: mdl-30372035
ABSTRACT
An electron paramagnetic resonance (EPR)-based method for noninvasive three-dimensional extracellular pH mapping was developed using a pH-sensitive nitroxyl radical as an exogenous paramagnetic probe. Fast projection scanning with a constant magnetic field sweep enabled the acquisition of four-dimensional (3D spatial +1D spectral) EPR images within 7.5 min. Three-dimensional maps of pH were reconstructed by processing the pH-dependent spectral information on the images. To demonstrate the proposed method of pH mapping, the progress of extracellular acidosis in tumor-bearing mouse legs was studied. Furthermore, extracellular pH mapping was used to visualize the spatial distribution of acidification in different tumor xenograft mouse models of human-derived pancreatic ductal adenocarcinoma cells. The proposed EPR-based pH mapping method enabled quantitative visualization of regional changes in extracellular pH associated with altered tumor metabolism.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Carcinoma de Células Escamosas / Carcinoma Ductal Pancreático / Imageamento Tridimensional Limite: Animals / Humans Idioma: En Revista: Anal Chem Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Japão
Texto completo
Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Carcinoma de Células Escamosas / Carcinoma Ductal Pancreático / Imageamento Tridimensional Limite: Animals / Humans Idioma: En Revista: Anal Chem Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Japão