Disulfide bridge formation to increase thermostability of DFPase enzyme: A computational study.
Comput Biol Chem
; 77: 272-278, 2018 Dec.
Article
em En
| MEDLINE
| ID: mdl-30396154
ABSTRACT
Organophosphate compounds bioremediation by use of organophosphorus degradation enzymes such as DFPase is a developing interest in industry and medicine. The most important problem with the bio-catalytic enzymes is their instability on high temperatures. This work carried out to find suitable locations for introducing disulfide bridges in DFPase enzyme. We employed some computational approaches to design the disulfide bridges and evaluate their roles in the enzyme structural thermostability. According to the in silico results, mutant 6 (V24C, C76) increased the enzyme thermostability relative to wild-type.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Hidrolases de Triester Fosfórico
/
Loligo
Limite:
Animals
Idioma:
En
Revista:
Comput Biol Chem
Assunto da revista:
BIOLOGIA
/
INFORMATICA MEDICA
/
QUIMICA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Irã