Quantification of DNA double-strand breaks using Geant4-DNA.
Med Phys
; 46(1): 405-413, 2019 Jan.
Article
em En
| MEDLINE
| ID: mdl-30418675
ABSTRACT
PURPOSE:
This study aims to standardize the simulation procedure in measuring DNA double-strand breaks (DSBs), by using advanced Monte Carlo toolkits, and newly introduced experimental methods for DNA DSB measurement.METHODS:
For the experimental quantification of DNA DSB, an innovative DNA dosimeter was used to produce experimental data. GATE in combination with Geant4-DNA toolkit were exploited to simulate the experimental environment. The PDB4DNA example of Geant4-DNA was upgraded and investigated. Parameters of the simulation such energy threshold (ET) for a strand break and base pair threshold (BPT) for a DSB were evaluated, depending on the dose.RESULTS:
Simulations resulted to minimum differentiation in comparison to experimental data for ET = 19 ± 1 eV and BPT = 10 bp, and high differentiation for ET<17.5 eV or ET>22.5 eV and BPT = 10 bp. There was also small differentiation for ET = 17.5 eV and BPT = 6 bp. Uncertainty has been kept lower than 3%.CONCLUSIONS:
This study includes first results on the quantification of DNA double-strand breaks. The energy spectrum of a LINAC was simulated and used for the first time to irradiate DNA molecules. Simulation outcome was validated on experimental data that were produced by a prototype DNA dosimeter.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Método de Monte Carlo
/
Quebras de DNA de Cadeia Dupla
Tipo de estudo:
Health_economic_evaluation
Idioma:
En
Revista:
Med Phys
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Grécia