On-site Method for Beef Detection Based on Strand Exchange Amplification.
Anal Sci
; 35(3): 337-341, 2019 Mar 10.
Article
em En
| MEDLINE
| ID: mdl-30449835
ABSTRACT
Meat screening plays a significant role in human health and religion. But the identification methods for beef were little reported. In this work, a simple colorimetric method based on denaturation bubble-mediated strand exchange amplification (SEA) was developed for the rapid and sensitive identification of beef. The whole strategy was performed on a portable metal bath and the distinguishable color between positive and negative controls was observed directly by the naked eyes. The feasibility using crude extraction samples by a heating treatment in PBS for 2 min was evaluated in duck spiked by beef. The result demonstrated that the developed method could identify as low as 1% (w/w) beef/duck within 50 min. Meanwhile, the results showed the method had a good repeatability and specificity. Therefore, this assay allows for the rapid, sensitive, specific detection of beef, and can be recommended as an effective, promising strategy for on-site meat identification.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Técnicas Biossensoriais
/
Qualidade dos Alimentos
/
Técnicas de Amplificação de Ácido Nucleico
/
Carne Vermelha
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Anal Sci
Ano de publicação:
2019
Tipo de documento:
Article