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Maternal Eed knockout causes loss of H3K27me3 imprinting and random X inactivation in the extraembryonic cells.
Inoue, Azusa; Chen, Zhiyuan; Yin, Qiangzong; Zhang, Yi.
Afiliação
  • Inoue A; Howard Hughes Medical Institute, Boston Children's Hospital, Boston, Massachusetts 02115, USA.
  • Chen Z; Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, Massachusetts 02115, USA.
  • Yin Q; Division of Hematology/Oncology, Department of Pediatrics, Boston Children's Hospital, Boston, Massachusetts 02115, USA.
  • Zhang Y; Howard Hughes Medical Institute, Boston Children's Hospital, Boston, Massachusetts 02115, USA.
Genes Dev ; 32(23-24): 1525-1536, 2018 12 01.
Article em En | MEDLINE | ID: mdl-30463900
Genomic imprinting is essential for mammalian development. Recent studies have revealed that maternal histone H3 Lys27 trimethylation (H3K27me3) can mediate DNA methylation-independent genomic imprinting. However, the regulatory mechanisms and functions of this new imprinting mechanism are largely unknown. Here we demonstrate that maternal Eed, an essential component of the Polycomb group complex 2 (PRC2), is required for establishing H3K27me3 imprinting. We found that all H3K27me3-imprinted genes, including Xist, lose their imprinted expression in Eed maternal knockout (matKO) embryos, resulting in male-biased lethality. Surprisingly, although maternal X-chromosome inactivation (XmCI) occurs in Eed matKO embryos at preimplantation due to loss of Xist imprinting, it is resolved at peri-implantation. Ultimately, both X chromosomes are reactivated in the embryonic cell lineage prior to random XCI, and only a single X chromosome undergoes random XCI in the extraembryonic cell lineage. Thus, our study not only demonstrates an essential role of Eed in H3K27me3 imprinting establishment but also reveals a unique XCI dynamic in the absence of Xist imprinting.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Histonas / Impressão Genômica / Inativação do Cromossomo X / Complexo Repressor Polycomb 2 Tipo de estudo: Etiology_studies Limite: Animals Idioma: En Revista: Genes Dev Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Histonas / Impressão Genômica / Inativação do Cromossomo X / Complexo Repressor Polycomb 2 Tipo de estudo: Etiology_studies Limite: Animals Idioma: En Revista: Genes Dev Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Estados Unidos