Sodium houttuyfonate inhibits LPSinduced mastitis in mice via the NFκB signalling pathway.
Mol Med Rep
; 19(3): 2279-2286, 2019 Mar.
Article
em En
| MEDLINE
| ID: mdl-30664199
ABSTRACT
Sodium houttuyfonate (SH) has been indicated to play an important antiinflammatory role. Previous studies have confirmed that SH can inhibit the NFκB pathway in lipopolysaccharide (LPS)induced mastitis in bovine mammary epithelial cells. However, the effects of SH on LPSinduced mastitis in animals should be verified to further evaluate its actual value. In the present study, the antiinflammatory effects of SH were investigated in mouse models and a mouse mammary epithelial cell line. Hematoxylin and eosin staining (H&E) showed that SH therapy significantly alleviated the pathological changes in mammary glands. Myeloperoxidase (MPO) activity analysis demonstrated that SH substantially decreased MPO activity in vivo. RTqPCR results showed that SH reduced the expression of interleukin (IL)1, IL6 and tumor necrosis factor α both in vivo and in vitro. In addition, western blot results indicated that SH suppressed the phosphorylation of nuclear factor kappalightchainenhancer of activated Bcells (NFκB) p65 protein and reduced the degradation of inhibitor of kappa light polypeptide gene enhancer in Bcells alpha protein in vivo and in vitro. These results demonstrated that SH ameliorates LPSinduced mastitis by inhibiting the NFκB pathway.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sulfitos
/
Alcanos
/
Fator de Transcrição RelA
/
Inflamação
/
Mastite
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2019
Tipo de documento:
Article