Identification of a post-transcriptional regulatory element in the human endogenous retroviral syncytin-1.
J Gen Virol
; 100(4): 662-668, 2019 04.
Article
em En
| MEDLINE
| ID: mdl-30794119
ABSTRACT
Retroviral transcripts have cis-acting elements that interact with host and viral proteins to enable efficient nuclear export and/or translation; however, it is poorly understood whether the transcripts of human endogenous retroviral genes retain such elements. Here, we show that human syncytin-1, which is derived from human endogenous retrovirus W, requires a 3' untranslated region (3'UTR) for efficient gene expression and retains a post-transcriptional regulatory element (named SPRE). The insertion of SPRE markedly increased a reporter gene (human immunodeficiency virus type 1 Gag) expression without affecting the amounts of nuclear or cytoplasmic transcript. Deletion analysis identified a required sequence for SPRE activity, and the prediction of the RNA secondary structure demonstrated a common secondary structure found among active SPRE sequences. Another human syncytin, syncytin-2, also requires a 3'UTR for efficient gene expression. These data provide insights into post-transcriptional regulation in endogenous retroviral gene expression.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas da Gravidez
/
Produtos do Gene env
/
Processamento de Proteína Pós-Traducional
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Retrovirus Endógenos
/
Elementos Reguladores de Transcrição
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
J Gen Virol
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Japão