Hydrazone formation of 2,4-dinitrophenylhydrazine with pyrroloquinoline quinone in porcine kidney diamine oxidase.
FEBS Lett
; 206(1): 111-4, 1986 Sep 29.
Article
em En
| MEDLINE
| ID: mdl-3093271
ABSTRACT
Homogeneous diamine oxidase (EC 1.4.3.6) from porcine kidney was treated with the inhibitor 2,4-dinitrophenylhydrazine (DNPH). The coloured compounds formed were detached with pronase and purified to homogeneity. When the reaction with DNPH was conducted under an O2 atmosphere, the product (obtained in a yield of 55%) was the C(5)-hydrazone of pyrroloquinoline quinone (PQQ) and DNPH, as revealed by its chromatographic behaviour, absorption spectrum and 1H-NMR spectrum. Only 6% of this hydrazone was formed under air, the main product isolated being an unidentified reaction product of DNPH with the enzyme. Porcine kidney diamine oxidase is the second mammalian enzyme shown to have PQQ as its prosthetic group. In view of the requirements for hydrazone formation with DNPH, it is incorrect to assume that inhibition of this type of enzymes with common hydrazines is simply due to blocking of the carbonyl group of its cofactor.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fenil-Hidrazinas
/
Amina Oxidase (contendo Cobre)
/
Hidrazonas
/
Rim
Limite:
Animals
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1986
Tipo de documento:
Article