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Macrophage migration inhibitory factor (MIF) controls cytokine release during respiratory syncytial virus infection in macrophages.
de Souza, Gabriela F; Muraro, Stéfanie P; Santos, Leonardo D; Monteiro, Ana Paula T; da Silva, Amanda G; de Souza, Ana Paula D; Stein, Renato T; Bozza, Patrícia T; Porto, Bárbara N.
Afiliação
  • de Souza GF; Laboratory of Clinical and Experimental Immunology, Infant Center, School of Medicine, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil.
  • Muraro SP; Laboratory of Clinical and Experimental Immunology, Infant Center, School of Medicine, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil.
  • Santos LD; Laboratory of Clinical and Experimental Immunology, Infant Center, School of Medicine, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil.
  • Monteiro APT; Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, RJ, Brazil.
  • da Silva AG; Laboratory of Clinical and Experimental Immunology, Infant Center, School of Medicine, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil.
  • de Souza APD; Laboratory of Clinical and Experimental Immunology, Infant Center, School of Medicine, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil.
  • Stein RT; Laboratory of Pediatric Respirology, Infant Center, School of Medicine, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil.
  • Bozza PT; Laboratory of Immunopharmacology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, RJ, Brazil.
  • Porto BN; Laboratory of Clinical and Experimental Immunology, Infant Center, School of Medicine, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil. barbara.porto@sickkids.ca.
Inflamm Res ; 68(6): 481-491, 2019 Jun.
Article em En | MEDLINE | ID: mdl-30944975
OBJECTIVE AND DESIGN: Respiratory syncytial virus (RSV) is the major cause of infection in children up to 2 years old and reinfection is very common among patients. Tissue damage in the lung caused by RSV leads to an immune response and infected cells activate multiple signaling pathways and massive production of inflammatory mediators like macrophage migration inhibitory factor (MIF), a pro-inflammatory cytokine. Therefore, we sought to investigate the role of MIF during RSV infection in macrophages. METHODS: We evaluated MIF expression in BALB/c mice-derived macrophages stimulated with different concentrations of RSV by Western blot and real-time PCR. Additionally, different inhibitors of signaling pathways and ROS were used to evaluate their importance for MIF expression. Furthermore, we used a specific MIF inhibitor, ISO-1, to evaluate the role of MIF in viral clearance and in RSV-induced TNF-α, MCP-1 and IL-10 release from macrophages. RESULTS: We showed that RSV induces MIF expression dependently of ROS, 5-LOX, COX and PI3K activation. Moreover, viral replication is necessary for RSV-triggered MIF expression. Differently, p38 MAPK in only partially needed for RSV-induced MIF expression. In addition, MIF is important for the release of TNF-α, MCP-1 and IL-10 triggered by RSV in macrophages. CONCLUSIONS: In conclusion, we demonstrate that MIF is expressed during RSV infection and controls the release of pro-inflammatory cytokines from macrophages in an in vitro model.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Citocinas / Fatores Inibidores da Migração de Macrófagos / Infecções por Vírus Respiratório Sincicial / Macrófagos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Inflamm Res Assunto da revista: ALERGIA E IMUNOLOGIA / PATOLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Citocinas / Fatores Inibidores da Migração de Macrófagos / Infecções por Vírus Respiratório Sincicial / Macrófagos Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Inflamm Res Assunto da revista: ALERGIA E IMUNOLOGIA / PATOLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil