Identification and Expression Analysis of Chitinase Genes in Pitchers of Nepenthes sp. during Development.
Dokl Biochem Biophys
; 484(1): 29-32, 2019 May.
Article
em En
| MEDLINE
| ID: mdl-31012007
ABSTRACT
Fifteen chitinases of classes I-V were identified in the transcriptomes of pitchers and adult leaves of the carnivorous plant Nepenthes sp. Ten of these chitinases were identified for the first time, including the chitinases of classes II and V. The expression levels of all found chitinase genes in leaves and at three stages of pitcher development were determined. The maximum level of transcriptional activity in an open pitcher was observed for the genes encoding chitinase NChi4 (class II) and its isoforms. The expression levels of these genes significantly increased as the pitcher developed. In addition, for the first time, transcription of the genes encoding chitinases of all five classes was detected in the leaves of this plant.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
/
Regulação Enzimológica da Expressão Gênica
/
Quitinases
/
Genes de Plantas
/
Regulação da Expressão Gênica de Plantas
/
Caryophyllales
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Dokl Biochem Biophys
Assunto da revista:
BIOFISICA
/
BIOQUIMICA
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Federação Russa