The impact of poly-A microsatellite heterologies in meiotic recombination.
Life Sci Alliance
; 2(2)2019 04.
Article
em En
| MEDLINE
| ID: mdl-31023833
ABSTRACT
Meiotic recombination has strong, but poorly understood effects on short tandem repeat (STR) instability. Here, we screened thousands of single recombinant products with sperm typing to characterize the role of polymorphic poly-A repeats at a human recombination hotspot in terms of hotspot activity and STR evolution. We show that the length asymmetry between heterozygous poly-A's strongly influences the recombination outcome:
a heterology of 10 A's (9A/19A) reduces the number of crossovers and elevates the frequency of non-crossovers, complex recombination products, and long conversion tracts. Moreover, the length of the heterology also influences the STR transmission during meiotic repair with a strong and significant insertion bias for the short heterology (6A/7A) and a deletion bias for the long heterology (9A/19A). In spite of this opposing insertion-/deletion-biased gene conversion, we find that poly-A's are enriched at human recombination hotspots that could have important consequences in hotspot activation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Poli A
/
Repetições de Microssatélites
/
Troca Genética
/
Heterozigoto
/
Meiose
Limite:
Humans
/
Male
Idioma:
En
Revista:
Life Sci Alliance
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Áustria