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An HRM Assay to Differentiate Sheeppox Virus Vaccine Strains from Sheeppox Virus Field Isolates and other Capripoxvirus Species.
Chibssa, Tesfaye Rufael; Settypalli, Tirumala Bharani K; Berguido, Francisco J; Grabherr, Reingard; Loitsch, Angelika; Tuppurainen, Eeva; Nwankpa, Nick; Tounkara, Karim; Madani, Hafsa; Omani, Amel; Diop, Mariane; Cattoli, Giovanni; Diallo, Adama; Lamien, Charles Euloge.
Afiliação
  • Chibssa TR; Animal Production and Health Laboratory, Joint FAO/IAEA Agricultural and Biotechnology laboratory, Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, A1400, Vienna, Austria.
  • Settypalli TBK; Institute of Biotechnology, University of Natural Resources and Life Sciences (BOKU), Muthgasse 18, 1190, Vienna, Austria.
  • Berguido FJ; National Animal Health Diagnostic and Investigation Center (NAHDIC), P.O. Box, 04, Sebeta, Ethiopia.
  • Grabherr R; Animal Production and Health Laboratory, Joint FAO/IAEA Agricultural and Biotechnology laboratory, Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, A1400, Vienna, Austria.
  • Loitsch A; Animal Production and Health Laboratory, Joint FAO/IAEA Agricultural and Biotechnology laboratory, Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, A1400, Vienna, Austria.
  • Tuppurainen E; Institute of Biotechnology, University of Natural Resources and Life Sciences (BOKU), Muthgasse 18, 1190, Vienna, Austria.
  • Nwankpa N; Institute for Veterinary Disease Control, Austrian Agency for Health and Food Safety (AGES), Mödling, Austria.
  • Tounkara K; Independent veterinary consultant, Helsinki, Finland.
  • Madani H; African Union Pan African Veterinary Vaccine Centre, (AU-PANVAC), P.O. Box 1746, Debre Ziet, Ethiopia.
  • Omani A; African Union Pan African Veterinary Vaccine Centre, (AU-PANVAC), P.O. Box 1746, Debre Ziet, Ethiopia.
  • Diop M; Institut National de la Médecine Vétérinaire, Laboratoire Central Vétérinaire d'Alger, Algiers, Algeria.
  • Cattoli G; Institut National de la Médecine Vétérinaire, Laboratoire Central Vétérinaire d'Alger, Algiers, Algeria.
  • Diallo A; Laboratoire National d'Elevage et de Recherches Vétérinaires, Institut Sénégalais de Recherches Agricoles (ISRA), BP 2057 Dakar-Hann, Dakar, Senegal.
  • Lamien CE; Animal Production and Health Laboratory, Joint FAO/IAEA Agricultural and Biotechnology laboratory, Division of Nuclear Techniques in Food and Agriculture, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, A1400, Vienna, Austria.
Sci Rep ; 9(1): 6646, 2019 04 30.
Article em En | MEDLINE | ID: mdl-31040355
ABSTRACT
Sheep poxvirus (SPPV), goat poxvirus (GTPV) and lumpy skin disease virus (LSDV) affect small ruminants and cattle causing sheeppox (SPP), goatpox (GTP) and lumpy skin disease (LSD) respectively. In endemic areas, vaccination with live attenuated vaccines derived from SPPV, GTPV or LSDV provides protection from SPP and GTP. As live poxviruses may cause adverse reactions in vaccinated animals, it is imperative to develop new diagnostic tools for the differentiation of SPPV field strains from attenuated vaccine strains. Within the capripoxvirus (CaPV) homolog of the variola virus B22R gene, we identified a unique region in SPPV vaccines with two deletions of 21 and 27 nucleotides and developed a High-Resolution Melting (HRM)-based assay. The HRM assay produces four distinct melting peaks, enabling the differentiation between SPPV vaccines, SPPV field isolates, GTPV and LSDV. This HRM assay is sensitive, specific, and provides a cost-effective means for the detection and classification of CaPVs and the differentiation of SPPV vaccines from SPPV field isolates.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças dos Ovinos / Vacinas Virais / Capripoxvirus / Reação em Cadeia da Polimerase em Tempo Real Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Áustria
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças dos Ovinos / Vacinas Virais / Capripoxvirus / Reação em Cadeia da Polimerase em Tempo Real Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Áustria