Your browser doesn't support javascript.
loading
Thermoregulation via Temperature-Dependent PGD2 Production in Mouse Preoptic Area.
Wang, Tongfei A; Teo, Chin Fen; Åkerblom, Malin; Chen, Chao; Tynan-La Fontaine, Marena; Greiner, Vanille Juliette; Diaz, Aaron; McManus, Michael T; Jan, Yuh Nung; Jan, Lily Y.
Afiliação
  • Wang TA; Departments of Physiology, Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Teo CF; Departments of Physiology, Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA; Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Åkerblom M; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94158, USA; UCSF Diabetes Center, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Chen C; Departments of Physiology, Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Tynan-La Fontaine M; Departments of Physiology, Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA; Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Greiner VJ; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94158, USA; UCSF Diabetes Center, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Diaz A; Department of Neurological Surgery, University of California, San Francisco, San Francisco, CA 94158, USA.
  • McManus MT; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94158, USA; UCSF Diabetes Center, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Jan YN; Departments of Physiology, Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA; Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Jan LY; Departments of Physiology, Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA; Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94158, USA. Electronic address: lily.jan@ucsf.edu.
Neuron ; 103(2): 309-322.e7, 2019 07 17.
Article em En | MEDLINE | ID: mdl-31151773
ABSTRACT
Body temperature control is essential for survival. In mammals, thermoregulation is mediated by the preoptic area of anterior hypothalamus (POA), with ∼30% of its neurons sensitive to brain temperature change. It is still unknown whether and how these temperature-sensitive neurons are involved in thermoregulation, because for eight decades they have only been identified via electrophysiological recording. By combining single-cell RNA-seq with whole-cell patch-clamp recordings, we identified Ptgds as a genetic marker for temperature-sensitive POA neurons. Then, we demonstrated these neurons' role in thermoregulation via chemogenetics. Given that Ptgds encodes the enzyme that synthesizes prostaglandin D2 (PGD2), we further explored its role in thermoregulation. Our study revealed that rising temperature of POA alters the activity of Ptgds-expressing neurons so as to increase PGD2 production. PGD2 activates its receptor DP1 and excites downstream neurons in the ventral medial preoptic area (vMPO) that mediates body temperature decrease, a negative feedback loop for thermoregulation.
Assuntos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Área Pré-Óptica / Temperatura / Regulação da Temperatura Corporal / Prostaglandina D2 / Neurônios Limite: Animals / Humans / Male Idioma: En Revista: Neuron Assunto da revista: NEUROLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Área Pré-Óptica / Temperatura / Regulação da Temperatura Corporal / Prostaglandina D2 / Neurônios Limite: Animals / Humans / Male Idioma: En Revista: Neuron Assunto da revista: NEUROLOGIA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Estados Unidos