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Carvacrol encapsulated nanocarrier/ nanoemulsion abrogates angiogenesis by downregulating COX-2, VEGF and CD31 in vitro and in vivo in a lung adenocarcinoma model.
Khan, Imran; Bhardwaj, Monika; Shukla, Shruti; Lee, Hoomin; Oh, Mi-Hwa; Bajpai, Vivek K; Huh, Yun Suk; Kang, Sun Chul.
Afiliação
  • Khan I; Department of Biological Engineering, Biohybrid Systems Research Center (BSRC), Inha University, 100 Inha-ro, Nam-gu, Incheon, 22212, Republic of Korea; Department of Biotechnology, Daegu University, Gyeongsan, Gyeongbuk, 38453, Republic of Korea.
  • Bhardwaj M; Laboratory of Biochemistry and cellular Engineering, Daegu Gyeongbuk Institute of Science and Technology, Daegu, 42988, Republic of Korea; Department of Biotechnology, Daegu University, Gyeongsan, Gyeongbuk, 38453, Republic of Korea.
  • Shukla S; Department of Energy and Materials Engineering, Dongguk University-Seoul, 30 Pildong-ro 1-gil, Seoul, 04620, Republic of Korea.
  • Lee H; Department of Biological Engineering, Biohybrid Systems Research Center (BSRC), Inha University, 100 Inha-ro, Nam-gu, Incheon, 22212, Republic of Korea.
  • Oh MH; Animal Production Research and Development Division, National Institute of Animal Science, Jeonju, 54875, Republic of Korea.
  • Bajpai VK; Department of Energy and Materials Engineering, Dongguk University-Seoul, 30 Pildong-ro 1-gil, Seoul, 04620, Republic of Korea. Electronic address: vbajpai04@yahoo.com.
  • Huh YS; Department of Biological Engineering, Biohybrid Systems Research Center (BSRC), Inha University, 100 Inha-ro, Nam-gu, Incheon, 22212, Republic of Korea. Electronic address: yunsuk.huh@inha.ac.kr.
  • Kang SC; Department of Biotechnology, Daegu University, Gyeongsan, Gyeongbuk, 38453, Republic of Korea. Electronic address: sckang@daegu.ac.kr.
Colloids Surf B Biointerfaces ; 181: 612-622, 2019 Sep 01.
Article em En | MEDLINE | ID: mdl-31202132
ABSTRACT
Nanoemulsion-based synthesis has been introduced to enhance the bioavailability of natural compounds at target sites for their various biomedical applications. In this study, we synthesized carvacrol nanoemulsion (CN) an oil-in-water (O/W) as a nano-emulsion vehicle system by using ultrasonication emulsification for anti-angiogenesis therapy formulated by combining MCT, lecithin, and polysorbate 80 at the O/W interface called carvacrol encapsulated nanoemulsion (CEN). The diameter of CEN determined by TEM analysis was 105.32 nm. The hydrodynamic droplet size was 101.0 nm with a -39.38-mV zeta potential. The stability of the synthesized CEN was approved till 100 days without any change in diameter size distribution and encapsulation efficiency. We evaluated the role of CEN on angiogenesis in lung adenocarcinoma A549 cells both in vitro and in vivo and observed that it reduced the growth and MMP levels of A549 cells in a dose-dependent manner. Exposure to CEN decreased the activation of MAPK p38 as well as ERK. Moreover, we found that CEN reduced the expression of VEGF and CD31 in A549 cells both in vitro and in vivo. Our in-silico study also indicated the binding of carvacrol to COX-2 and VEGF at the active and allosteric sites of CD31 with low binding energy. Overall, CEN induced anti-angiogenic effects in A549 cells in vitro, in silico, and in vivo, thereby establishing its potential as targeted drug delivery vehicle against angiogenesis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nanopartículas / Adenocarcinoma de Pulmão / Cimenos / Neoplasias Pulmonares / Neovascularização Patológica / Antineoplásicos Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: Colloids Surf B Biointerfaces Assunto da revista: QUIMICA Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nanopartículas / Adenocarcinoma de Pulmão / Cimenos / Neoplasias Pulmonares / Neovascularização Patológica / Antineoplásicos Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: Colloids Surf B Biointerfaces Assunto da revista: QUIMICA Ano de publicação: 2019 Tipo de documento: Article