Eukaryotic Base Excision Repair: New Approaches Shine Light on Mechanism.
Annu Rev Biochem
; 88: 137-162, 2019 06 20.
Article
em En
| MEDLINE
| ID: mdl-31220977
ABSTRACT
Genomic DNA is susceptible to endogenous and environmental stresses that modify DNA structure and its coding potential. Correspondingly, cells have evolved intricate DNA repair systems to deter changes to their genetic material. Base excision DNA repair involves a number of enzymes and protein cofactors that hasten repair of damaged DNA bases. Recent advances have identified macromolecular complexes that assemble at the DNA lesion and mediate repair. The repair of base lesions generally requires five enzymatic activities glycosylase, endonuclease, lyase, polymerase, and ligase. The protein cofactors and mechanisms for coordinating the sequential enzymatic steps of repair are being revealed through a range of experimental approaches. We discuss the enzymes and protein cofactors involved in eukaryotic base excision repair, emphasizing the challenge of integrating findings from multiple methodologies. The results provide an opportunity to assimilate biochemical findings with cell-based assays to uncover new insights into this deceptively complex repair pathway.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Genoma
/
DNA Glicosilases
/
DNA Polimerase Dirigida por DNA
/
Endonucleases
/
Ligases
/
Liases
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Annu Rev Biochem
Ano de publicação:
2019
Tipo de documento:
Article