Cytotoxicity and proinflammatory effects of titanium and zirconia particles.
Int J Implant Dent
; 5(1): 25, 2019 Jul 09.
Article
em En
| MEDLINE
| ID: mdl-31286286
ABSTRACT
BACKGROUND:
To assess the effects of differently sized titanium (Ti) and zirconia (Zr) particles on (1) the metabolic activity of osteosarcoma-derived osteoblasts (SaOs-2) and human gingival fibroblasts (HGF) and (2) the cytokine expression of monocytes (THP-1)METHODS:
Ti (60-80 nm and 100 nm) and Zr (2 µm and 75 µm) particles were incubated with SaOs-2, HGF, and THP-1 cells. At days 0, 2, 4, and 7 and 0, 1, 2, and 4 (THP-1), the mitochondrial activity was assessed and enzyme-linked immunosorbent assays were used to determine interleukin (IL)-1 beta and IL-6 concentrations of stimulated THP-1 at day 1.RESULTS:
Ti60-80, Ti100, Zr2, and Zr75 particles were associated with gradual and significant within-group decreases in the viability of SaOs-2 and HGF cells. These effects were less pronounced in the Zr group. Similar to control cells, THP-1 did not reveal any significant increases in IL-1 beta and IL-6 concentrations. Viability of THP-1 was merely impaired in the presence of Ti100.CONCLUSIONS:
Ti and Zr particles had a detrimental effect on the viability of SaOs-2 and HGF, but no proinflammatory effect on THP-1.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Int J Implant Dent
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Alemanha