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Molecular Basis for Control of Diverse Genome Stability Factors by the Multi-BRCT Scaffold Rtt107.
Wan, Bingbing; Wu, Jian; Meng, Xiangzhou; Lei, Ming; Zhao, Xiaolan.
Afiliação
  • Wan B; Molecular Biology Department, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.
  • Wu J; Shanghai Institute of Precision Medicine, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200125, China.
  • Meng X; Molecular Biology Department, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.
  • Lei M; Shanghai Institute of Precision Medicine, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200125, China; Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine, Shanghai 200125, China
  • Zhao X; Molecular Biology Department, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA. Electronic address: zhaox1@mskcc.org.
Mol Cell ; 75(2): 238-251.e5, 2019 07 25.
Article em En | MEDLINE | ID: mdl-31348879
BRCT domains support myriad protein-protein interactions involved in genome maintenance. Although di-BRCT recognition of phospho-proteins is well known to support the genotoxic response, whether multi-BRCT domains can acquire distinct structures and functions is unclear. Here we present the tetra-BRCT structures from the conserved yeast protein Rtt107 in free and ligand-bound forms. The four BRCT repeats fold into a tetrahedral structure that recognizes unmodified ligands using a bi-partite mechanism, suggesting repeat origami enabling function acquisition. Functional studies show that Rtt107 binding of partner proteins of diverse activities promotes genome replication and stability in both distinct and concerted manners. A unified theme is that tetra- and di-BRCT domains of Rtt107 collaborate to recruit partner proteins to chromatin. Our work thus illustrates how a master regulator uses two types of BRCT domains to recognize distinct genome factors and direct them to chromatin for constitutive genome protection.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Proteínas Nucleares / Proteínas de Saccharomyces cerevisiae / Instabilidade Genômica / Domínios e Motivos de Interação entre Proteínas Idioma: En Revista: Mol Cell Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Proteínas Nucleares / Proteínas de Saccharomyces cerevisiae / Instabilidade Genômica / Domínios e Motivos de Interação entre Proteínas Idioma: En Revista: Mol Cell Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Estados Unidos