Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2.

Zeng, Ruizhu; Sidik, Harwin; Robinson, Kim S; Zhong, Franklin L; Reversade, Bruno; Pouladi, Mahmoud A

Zeng, Ruizhu; Sidik, Harwin; Robinson, Kim S; Zhong, Franklin L; Reversade, Bruno; Pouladi, Mahmoud A.

Afiliação

Zeng R; Translational Laboratory in Genetic Medicine, Agency for Science, Technology and Research, Singapore (A*STAR), 8A Biomedical Grove, Immunos, Level 5, 138648, Singapore.
Sidik H; Translational Laboratory in Genetic Medicine, Agency for Science, Technology and Research, Singapore (A*STAR), 8A Biomedical Grove, Immunos, Level 5, 138648, Singapore.
Robinson KS; Institute of Medical Biology, A*STAR, Immunos, 138648, Singapore; Skin Research Institute of Singapore, Immunos, 138648, Singapore.
Zhong FL; Institute of Molecular and Cell Biology, A*STAR, Proteos, 138673, Singapore.; Institute of Medical Biology, A*STAR, Immunos, 138648, Singapore; Skin Research Institute of Singapore, Immunos, 138648, Singapore; Lee Kong Chian School of Medicine, Nanyang Technological University, 636921, Singapore.
Reversade B; Institute of Molecular and Cell Biology, A*STAR, Proteos, 138673, Singapore.; Institute of Medical Biology, A*STAR, Immunos, 138648, Singapore; Department of Paediatrics, National University of Singapore, 1E Kent Ridge Road, 119228, Singapore; Medical Genetics Department, Koç University School of Me
Pouladi MA; Translational Laboratory in Genetic Medicine, Agency for Science, Technology and Research, Singapore (A*STAR), 8A Biomedical Grove, Immunos, Level 5, 138648, Singapore; Department of Medicine, National University of Singapore, 117597, Singapore; Department of Physiology, National University of Singa

Stem Cell Res ; 40: 101533, 2019 10.

Article em En | MEDLINE | ID: mdl-31450191

ABSTRACT

ABSTRACT

Rett syndrome (RTT) is a childhood neurodevelopmental disorder caused by mutations in MECP2. To study the molecular mechanisms underlying RTT, four sublines of H1 hESCs were generated, carrying a hemizygous knockout or mutant allele of MECP2. Exons 3 and 4 of MECP2 were targeted using the CRISPR/Cas9 nuclease system.

Assuntos

Células-Tronco Embrionárias/citologia; Edição de Genes; Proteína 2 de Ligação a Metil-CpG/genética; Sistemas CRISPR-Cas/genética; Diferenciação Celular; Linhagem Celular; Células-Tronco Embrionárias/metabolismo; Éxons; Humanos; Cariótipo; Síndrome de Rett/genética; Síndrome de Rett/patologia

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteína 2 de Ligação a Metil-CpG / Células-Tronco Embrionárias / Edição de Genes Limite: Humans Idioma: En Revista: Stem Cell Res Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Singapura

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