Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2.
Stem Cell Res
; 40: 101533, 2019 10.
Article
em En
| MEDLINE
| ID: mdl-31450191
ABSTRACT
Rett syndrome (RTT) is a childhood neurodevelopmental disorder caused by mutations in MECP2. To study the molecular mechanisms underlying RTT, four sublines of H1 hESCs were generated, carrying a hemizygous knockout or mutant allele of MECP2. Exons 3 and 4 of MECP2 were targeted using the CRISPR/Cas9 nuclease system.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteína 2 de Ligação a Metil-CpG
/
Células-Tronco Embrionárias
/
Edição de Genes
Limite:
Humans
Idioma:
En
Revista:
Stem Cell Res
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Singapura