Hybrid genome assembly and annotation of Danionella translucida.
Sci Data
; 6(1): 156, 2019 08 26.
Article
em En
| MEDLINE
| ID: mdl-31451709
ABSTRACT
Studying neuronal circuits at cellular resolution is very challenging in vertebrates due to the size and optical turbidity of their brains. Danionella translucida, a close relative of zebrafish, was recently introduced as a model organism for investigating neural network interactions in adult individuals. Danionella remains transparent throughout its life, has the smallest known vertebrate brain and possesses a rich repertoire of complex behaviours. Here we sequenced, assembled and annotated the Danionella translucida genome employing a hybrid Illumina/Nanopore read library as well as RNA-seq of embryonic, larval and adult mRNA. We achieved high assembly continuity using low-coverage long-read data and annotated a large fraction of the transcriptome. This dataset will pave the way for molecular research and targeted genetic manipulation of this novel model organism.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cyprinidae
/
Genoma
/
Anotação de Sequência Molecular
Limite:
Animals
Idioma:
En
Revista:
Sci Data
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Alemanha