Transcriptional regulation of the Salmonella enterica std fimbrial operon by the RcsCDB system.
Microbiology (Reading)
; 165(11): 1245-1250, 2019 11.
Article
em En
| MEDLINE
| ID: mdl-31486760
ABSTRACT
In Salmonella enterica serovar Typhimurium, the RcsCDB regulatory system controls the expression of genes involved in synthesis of colanic acid, formation of flagella and virulence. Here, we show that activation of the RcsCDB system downregulates expression of std, an operon that encodes fimbriae involved in Salmonella attachment to the mucus layer in the large intestine. Bioinformatic analysis predicts the existence of an RcsB-binding site located 180 bp upstream to the +1 transcription start site of the std promoter, and electrophoretic mobility shift assays confirm that RcsB binds the std promoter region in vitro. This study adds RcsB to the list of regulators of std transcription and provides an example of modulation of fimbriae synthesis by a signal transduction system.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Salmonella typhimurium
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Proteínas de Bactérias
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Transdução de Sinais
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Regulação Bacteriana da Expressão Gênica
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Fímbrias Bacterianas
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Proteínas de Fímbrias
Idioma:
En
Revista:
Microbiology (Reading)
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Argentina