Photobiomodulation Increases Viability in Full-Thickness Grafts in Rats Submitted to Nicotine.
Lasers Surg Med
; 52(5): 449-455, 2020 06.
Article
em En
| MEDLINE
| ID: mdl-31493338
ABSTRACT
BACKGROUND AND OBJECTIVES:
Photobiomodulation (PBM) therapy with 830 nm wavelength or 660 wavelength to compare the effects with parameters of 30 mW, 0.028 cm2 , 9.34 seconds, and 3.64 J on the total integration of total skin grafts in rats submitted to nicotine. STUDY DESIGN/MATERIALS ANDMETHODS:
Sixty male Wistar rats were divided in six groups Sham-skin-grafting surgery; 830 nm-skin-grafting followed by 830 nm irradiation; 660 nm-skin grafting followed by 660 nm irradiation; Nicotine-subjected to subcutaneous nicotine injection (2 mg/kg twice a day for 4 weeks), followed by skin grafting; Group Nicotine/830 nm-similar to Group Nicotine, followed by 830 nm irradiation; Group Nicotine/660 nm-similar to Group Nicotine, followed by 660 nm irradiation. The percentage contraction of the grafting tissue was evaluated through ImageJ®. The thickness of the epidermis, inflammatory infiltrates, and the space between the implanted tissue and receptor bed were determined by histology; and the expression of vascular growth factor and blood vessel density (factor VIII) were evaluated by immunohistochemistry.RESULTS:
The PBM at both wavelengths promoted a facilitating effect on the integration of the skin graft under nicotine and had a more significant effect on the thickness of the epidermis and expression of angiogenesis without nicotine at a wavelength of 830 nm. Different wavelengths influence responses related to the viability of cutaneous grafts in rats submitted to nicotine.CONCLUSIONS:
The PBM with 830 nm and 660 nm promoted beneficial results in skin grafts submitted to the deleterious action of nicotine. Lasers Surg. Med. © 2019 Wiley Periodicals, Inc.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cicatrização
/
Transplante de Pele
/
Terapia com Luz de Baixa Intensidade
/
Estimulantes Ganglionares
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Sobrevivência de Enxerto
/
Nicotina
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Lasers Surg Med
Ano de publicação:
2020
Tipo de documento:
Article