Generation of a neutralizing antibody against RD114-pseudotyped viral vectors.
J Gen Virol
; 101(9): 1008-1018, 2020 09.
Article
em En
| MEDLINE
| ID: mdl-31702531
ABSTRACT
The feline endogenous RD114 glycoprotein has proved to be an attractive envelope to pseudotype both retroviral and lentiviral vectors. As a surface protein, its detection on packaging cells as well as viral particles would be useful in different fields of its use. To address this, we generated a monoclonal antibody against RD114 by immunization of rats, termed 22F10. Once seroconversion was confirmed, purified 22F10 was cloned into murine Fc and characterized with a binding affinity of 10nM. The antibody was used to detect RD114 and its variant envelopes on different stable viral packaging cell lines (FLYRD18 and WinPac-RD). 22F10 was also shown to prevent the infections of different strains of RD-pseudotyped vectors but not related envelope glycoproteins by blocking cell surface receptor binding. We are the first to report the neutralization of viral particles by a monoclonal αRD114 antibody.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas dos Retroviridae
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Proteínas do Envelope Viral
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Anticorpos Neutralizantes
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Vetores Genéticos
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Anticorpos Monoclonais
Limite:
Animals
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Humans
Idioma:
En
Revista:
J Gen Virol
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Reino Unido