Your browser doesn't support javascript.
loading
Co-existence of Citrobacter freundii exacerbated Pseudomonas aeruginosa infection in vivo.
Peng, Qing; Chen, Lin; Zhou, Shuqin; Li, Haiyan; Long, Jun; Yao, Fen; Zhuang, Yijing; Zhang, Zijie; Huang, Yuanchun; Duan, Kangmin.
Afiliação
  • Peng Q; Department of Hepatobiliary Surgery II, Institute of Regenerative Medicine, Zhujiang Hospital of Southern Medical University, Guangzhou, China; Department of Medical Microbiology and Infectious Diseases & Oral Biology, Rady Faculty of Health Sciences, University of Manitoba, 780 Bannatyne Ave, W
  • Chen L; Department of Medical Microbiology and Infectious Diseases & Oral Biology, Rady Faculty of Health Sciences, University of Manitoba, 780 Bannatyne Ave, Winnipeg, MB R3E 0W2, Canada; Faculty of Life Sciences, Northwest University, 229 Taibai Rd. North, Xi'an, Shaanxi, China.
  • Zhou S; Department of Anesthesiology, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong, China.
  • Li H; Department of Pharmacology, Shantou University Medical College, Shantou, Guangdong, China.
  • Long J; Department of Clinical Laboratory, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong, China.
  • Yao F; Department of Pharmacology, Shantou University Medical College, Shantou, Guangdong, China.
  • Zhuang Y; Department of Clinical Laboratory, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong, China.
  • Zhang Z; Department of Pharmacology, Shantou University Medical College, Shantou, Guangdong, China.
  • Huang Y; Department of Clinical Laboratory, the first affiliated hospital of Shantou University Medical College, Shantou, Guangdong, China.
  • Duan K; Department of Medical Microbiology and Infectious Diseases & Oral Biology, Rady Faculty of Health Sciences, University of Manitoba, 780 Bannatyne Ave, Winnipeg, MB R3E 0W2, Canada. Electronic address: Kangmin.Duan@umanitoba.ca.
Int J Med Microbiol ; 310(1): 151379, 2020 Jan.
Article em En | MEDLINE | ID: mdl-31759864
The presence of bacterial species other than the pathogen at infection site can affect the progression of a bacterial infection. Based on the fact that Citrobacter freundii can coexist during Pseudomonas aeruginosa infection, this study aims to investigate the impact of the co-existing C. freundii on the pathogenesis of P. aeruginosa infection. A murine peritonitis model was used to compare the mortality rates and histopathology of P. aeruginosaPAO1 infection in the presence and absence of a C. freundii clinical isolate C9. We also investigated the intercellular interaction between PAO1 and C9 by examining pyocyanin production and comparing gene expression levels. The results demonstrate that co-infection with C9 significantly increased the mortality rate and tissue damages in PAO1 infected mice. At an inoculum of 106 CFU, no mortality was observed in the C9 infected group at three days post-infection, whereas the mortality rate in the PAO1-C9 co-infection group was 64%, compared with 24% in the PAO1 infected group. Pyocyanin production in P. aeruginosa PAO1 increased 8 folds approximately in the presence of C. freundii C9, and operons associated with phenazine synthesis, phzA1 and phzA2, were also upregulated. Disruption of the phzA1 and phzA2 eliminated the exacerbated pathogenicity in the co-infection group, indicating that the elevated pyocyanin production was the main contributing factor. The results suggest that co-existing C. freundii during P. aeruginosa infection can exacerbate the pathogenicity, which may have significant implications in patients infected with these bacteria.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peritonite / Infecções por Pseudomonas / Infecções por Enterobacteriaceae / Coinfecção Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Int J Med Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peritonite / Infecções por Pseudomonas / Infecções por Enterobacteriaceae / Coinfecção Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Int J Med Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2020 Tipo de documento: Article